Alternative respiratory pathway under drought is partially mediated by hydrogen peroxide and contributes to antioxidant protection in wheat leaves
Alternative respiratory pathway under drought is partially mediated by hydrogen peroxide and contributes to antioxidant protection in wheat leaves
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干旱下的替代呼吸途径部分由过氧化氢介导，有助于小麦叶片的抗氧化保护
DOI：
10.1626/pps.11.59
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Han, Na
中科院分区：
文献类型：
--
作者：
Feng, Hanqing;Duan, Jiangong;Han, Na
水分胁迫显著提高了小麦(Triticum aestivum L.)交替呼吸途径的能力，诱导了AOX1转录本的表达。树叶。用1 mM水杨基异羟肟酸(SHAM)处理的水分胁迫幼苗比单独干旱或假处理的幼苗产生更高水平的ROS。这一观察表明，在干旱条件下，抗氰化物呼吸可能在抗氧化保护方面发挥作用。外源过氧化氢处理有效地提高了交替呼吸途径的能力，诱导了AOX1转录。ROS清除剂4，5-二羟基-1，3-苯二磺酸(TIron)和二甲基硫脲(DMTU)可抑制水分胁迫下ROS的增加，并部分抑制抗氰呼吸和AOX1转录本的诱导。这些结果表明，干旱条件下抗氰化物呼吸的增强可能部分是由过氧化氢介导的。
Water stress significantly enhanced the capacity of alternative respiratory pathway and induced AOX1 transcript in wheat (Triticum aestivum L.) leaves. The water-stressed seedlings pretreated with 1 mM salicylhydroxamic acid (SHAM) had higher level of production of reactive oxygen species (ROS) than the seedlings either subjected to drought or SHAM treatment alone did. This observation suggests that cyanide-resistant respiration could play a role in antioxidant protection tinder the condition of drought. Exogenous application of hydrogen peroxide effectively increased the capacity of alternative respiratory pathway and induced AOX1 transcription. Pretreatment with ROS scavengers, such as 4,5-dihydroxy-1,3-benzene disulfonic acid (Tiron) and dim ethyl thiourea (DMTU), arrested the increase of ROS and partly inhibited the induction of both cyanide-resistant respiration and AOX1 transcript under water stress. These results suggest that the enhancement of cyanide-resistant respiration under drought might be partially mediated by hydrogen peroxide.