The femR315 gene from Staphylococcus aureus, the interruption of which results in reduced methicillin resistance, encodes a phosphoglucosamine mutase
The femR315 gene from Staphylococcus aureus, the interruption of which results in reduced methicillin resistance, encodes a phosphoglucosamine mutase
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DOI：
10.1128/jb.179.17.5321-5325.1997
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发表时间：
1997-09-01
影响因子：
3.2
通讯作者：
Tomasz, A
中科院分区：
文献类型：
--
作者：
Jolly, L;Wu, SW;Tomasz, A
femR 315基因最近通过Tn 551插入突变被鉴定为新的辅助基因之一，其改变导致金黄色葡萄球菌菌株COL.femR315的甲氧西林抗性急剧降低（也称为femI）理论上编码451个氨基酸的蛋白质，显示出与磷酸葡萄糖变位酶和催化以下异构化的类似酶的显著氨基酸序列同源性：己糖和己糖胺磷酸盐（S. Wu，H. de Lencastre，A. Sali和A. Tomasz，Microb. Drug Resist，2：277-286，1996）。我们在此描述了FemR 315蛋白的过量生产和纯化以及其作为磷酸葡糖胺的鉴定，其催化从葡糖胺-6-磷酸形成葡糖胺-1-磷酸，这是导致必需的肽聚糖前体UDP-N-乙酰葡糖胺的反应序列中的第一步。基于这些发现，我们建议将名称femR 315和femD更改为功能上更合适的名称glmM。
The femR315 gene was recently identified by Tn551 insertional mutagenesis as one of the new auxiliary genes, the alteration of which resulted in a drastically reduced methicillin resistance of the Staphylococcus aureus strain COL. femR315 (also known as femI) theoretically encoded a protein of 451 amino acids showing significant amino acid sequence homology with phosphoglucomutases and similar enzymes catalyzing the isomerization of hexoses and hexosamine phosphates (S. Wu, H. de Lencastre, A. Sali, and A. Tomasz, Microb. Drug Resist, 2:277-286, 1996), We describe here the overproduction and purification of the FemR315 protein as well as its identification as the phosphoglucosamine mutase which catalyzes the formation of glucosamine-1-phosphate from glucosamine-6-phosphate, the first step in the reaction sequence leading to the essential peptidoglycan precursor UDP-N-acetylglucosamine. On the basis of these findings, we propose to change the names femR315 and femD to the functionally more appropriate name glmM.