Unique gene expression signatures of independently-derived human embryonic stem cell lines
Unique gene expression signatures of independently-derived human embryonic stem cell lines
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DOI：
10.1093/hmg/ddh068
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发表时间：
2004-03-15
影响因子：
3.5
通讯作者：
Firpo, MT
中科院分区：
文献类型：
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作者：
Abeyta, MJ;Clark, AT;Firpo, MT
人类胚胎干细胞（human embryonic stem cells，hESC）具有分化为多种细胞类型的潜能。这种能力赋予hESC开发新疗法的希望，以及开发严格的遗传系统以探测人类基因功能的希望。然而，尽管人类胚胎干细胞即将用于临床和基础应用，但对其基本特性知之甚少。最近的报道已经记录了小鼠胚胎干细胞（mESC），成体干细胞和单个hESC系H9的转录谱。然而，迄今为止，独立衍生的hESC系的转录谱尚未进行比较。为了研究多个hESC系的相似性和差异性，我们比较了HSF-1、HSF-6和H9系的基因表达谱。我们发现，大多数检测的基因在所有三个细胞系中表达。然而，我们也观察到每个细胞系都有一个独特的表达特征，许多基因的表达仅限于一个或两个hESC细胞系。我们认为，观察到的独立衍生的人胚胎干细胞株之间的基因表达差异可能反映了每个株系的初始培养和/或该株系衍生的胚胎的潜在遗传学的固有差异。
Human embryonic stem cells (hESCs) have the potential to differentiate to diverse cell types. This ability endows hESCs with promise for the development of novel therapeutics, as well as promise for the development of a rigorous genetic system to probe human gene function. However, in spite of the impending utility of hESCs for clinical and basic applications, little is known about their fundamental properties. Recent reports have documented transcriptional profiles of mouse embryonic stem cells (mESCs), adult stem cells and a single hESC line, H9. To date, however, the transcriptional profiles of independently-derived hESC lines have not been compared. In order to examine the similarities and differences in multiple hESC lines, we compared gene expression profiles of the HSF-1, HSF-6 and H9 lines. We found that the majority of genes examined were expressed in all three cell lines. However, we also observed that each line possessed a unique expression signature; the expression of many genes was limited to just one or two hESC lines. We suggest that the observed differences in gene expression between independently-derived hESC lines may reflect inherent differences in the initial culture of each line and/or the underlying genetics of the embryos from which the lines were derived.