Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16-Monocytes A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16-Monocytes A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
复制标题
DOI：
10.1002/art.38860
复制
发表时间：
2014-12-01
影响因子：
13.3
通讯作者：
Rojas, M.
中科院分区：
文献类型：
--
作者：
Burbano, C.;Vasquez, G.;Rojas, M.
Objective.在各种慢性炎症过程中，单核细胞亚群的比例和数量都发生了变化。在系统性红斑狼疮（SLE）中，这一点尚未明确确定。对SLE患者、其他自身免疫性疾病患者和健康对照者的单核细胞亚群进行了评价。同时观察了非经典单核细胞和凋亡细胞对CD 14-CD 16单核细胞分化和功能的影响。方法.单核细胞亚群来自SLE患者（n 88），其他自身免疫性疾病的患者（n 37），和健康对照组（n 61）的血液样本分离的荧光激活细胞分选。为了评估CD 14-CD 16单核细胞和AC对CD 14-CD 16单核细胞分化的影响，我们开发了高度纯化的分选的单核细胞亚群的共培养模型，其在存在或不存在AC的情况下用确定比例的CD 14-CD 16和CD 14-CD 16单核细胞重建。分化成巨噬细胞后，加入CD 3淋巴细胞，并评价增殖细胞和CD 3 IFN细胞。使用细胞因子珠阵列板测试共培养物上清液。结果活动期SLE患者CD 14、CD 16单核细胞减少。SLE患者单核细胞HLA-DR表达降低，结合和吞噬AC的能力降低。在健康对照中，但在SLE患者中，用CD 14 CD 16单核细胞衍生的巨噬细胞治疗可减少T细胞增殖和CD 3 IFN细胞增殖，并增加肿瘤坏死因子、白细胞介素-10（IL-10）和IL-1的积累。结论我们的研究结果表明，CD 14-CD 16单核细胞，一个人口减少和非功能性SLE患者，有调节作用的CD 14-CD 16单核细胞和T细胞。
Objective. In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and healthy controls were evaluated. The effects of nonclassic monocytes and apoptotic cells (ACs) on the differentiation and function of CD14 CD16 monocytes were also studied. Methods. Monocyte subpopulations derived from the blood samples of SLE patients (n 88), patients with other autoimmune diseases (n 37), and healthy control subjects (n 61) were separated by fluorescence-activated cell sorting. To evaluate the effect of CD14 CD16 monocytes and ACs on the differentiation of CD14 CD16 monocytes, we developed a coculture model of highly purified sorted monocyte subpopulations, which were reconstituted with defined proportions of CD14 CD16 and CD14 CD16 monocytes in the presence or absence of ACs. After differentiation into macrophages, CD3 lymphocytes were added, and the proliferating cells and CD3 IFN cells were evaluated. A cytokine bead array panel was used to test the coculture supernatants. Results. There was a reduction in CD14 CD16 monocytes in patients with active SLE. Monocytes from SLE patients had decreased expression of HLA-DR and decreased ability to bind and phagocytize ACs. In healthy controls, but not SLE patients, treatment with macrophages derived from CD14 CD16 monocytes reduced T cell proliferation and proliferating CD3 IFN cells and increased the accumulation of tumor necrosis factor , interleukin-10 (IL-10), and IL-1 . Conclusion. Our findings show that CD14 CD16 monocytes, a population that is reduced and nonfunctional in SLE patients, have modulatory effects on CD14 CD16 monocytes and T cells.