Population structure and associated phenotypes of Salmonella enterica serovars Derby and Mbandaka overlap with host range.
Population structure and associated phenotypes of Salmonella enterica serovars Derby and Mbandaka overlap with host range.
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DOI：
10.1186/s12866-016-0628-4
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发表时间：
2016-02-04
期刊：
影响因子：
4.2
通讯作者：
Woodward MJ
中科院分区：
文献类型：
--
作者：
Hayward MR;Petrovska L;Jansen VA;Woodward MJ
在英国，肠沙门氏菌德比血清型经常从猪和火鸡中分离出来，而姆班达卡血清型则经常从牛、鸡和动物饲料中分离出来。通过比较基因组学、表型组学和突变体构建，我们之前提出了这些血清型表现出明显不同的宿主范围的可能机制原因。在这里，我们研究了英国这两种血清型的遗传和表型多样性。我们对 2000 年至 2010 年间从英国各地获得的 S. Derby 和 S. Mbandaka 分离株进行了系统发育重建，并进行了多项生化测定。我们表明，英国 S. Mbandaka 分离株由一种克隆谱系组成，该谱系适合在环境条件下熟练利用大豆中发现的代谢物。我们还表明，这种克隆谱系在 25°C 下形成生物膜，这表明这种血清型可能很好地适应离体生存，在动物饲料中生长。相反，我们表明 S. Derby 由两个不同的谱系组成：L1 和 L2。这些谱系在基因型和表型上有所不同，根据 SPI-23 的存在与否以及更有效地侵入猪空肠来源的细胞系 IPEC-J2 的能力来划分。这项研究的结果支持了以下假设：S. Derby 和 S. Mbandaka 宿主范围的差异是对发病机制、环境持久性以及对各自宿主环境中丰富代谢物的利用的适应。本文的在线版本 (doi:10.1186/s12866-016-0628-4) 包含补充材料，可供授权用户使用。
The Salmonella enterica serovar Derby is frequently isolated from pigs and turkeys whereas serovar Mbandaka is frequently isolated from cattle, chickens and animal feed in the UK. Through comparative genomics, phenomics and mutant construction we previously suggested possible mechanistic reasons why these serovars demonstrate apparently distinct host ranges. Here, we investigate the genetic and phenotypic diversity of these two serovars in the UK. We produce a phylogenetic reconstruction and perform several biochemical assays on isolates of S. Derby and S. Mbandaka acquired from sites across the UK between the years 2000 and 2010. We show that UK isolates of S. Mbandaka comprise of one clonal lineage which is adapted to proficient utilisation of metabolites found in soya beans under ambient conditions. We also show that this clonal lineage forms a biofilm at 25 °C, suggesting that this serovar maybe well adapted to survival ex vivo, growing in animal feed. Conversely, we show that S. Derby is made of two distinct lineages, L1 and L2. These lineages differ genotypically and phenotypically, being divided by the presence and absence of SPI-23 and the ability to more proficiently invade porcine jejunum derived cell line IPEC-J2. The results of this study lend support to the hypothesis that the differences in host ranges of S. Derby and S. Mbandaka are adaptations to pathogenesis, environmental persistence, as well as utilisation of metabolites abundant in their respective host environments. The online version of this article (doi:10.1186/s12866-016-0628-4) contains supplementary material, which is available to authorized users.