Characterization of Brucella suis clpB and clpAB mutants and participation of the genes in stress responses
Characterization of Brucella suis clpB and clpAB mutants and participation of the genes in stress responses
复制标题
DOI：
10.1128/jb.183.8.2677-2681.2001
复制
发表时间：
2001-04-01
影响因子：
3.2
通讯作者：
Köhler, S
中科院分区：
文献类型：
--
作者：
Ekaza, E;Teyssier, J;Köhler, S
病原体经常在宿主体内遇到压力条件。为了表征猪布鲁氏菌的应激反应，从猪布鲁氏菌中分离出与大肠杆菌 clpB 高度同源的基因，推导的氨基酸序列显示了应激反应蛋白 ClpB ATPase 家族的典型特征。在高温胁迫条件下，猪芽孢杆菌的 ClpB 被诱导，并且同基因猪芽孢杆菌 clpB 突变体表现出对高温、乙醇胁迫和酸性 pH 值的敏感性增加。通过互补，效果是可逆的。 clpA 和 clpB 同时失活会产生对氧化应激敏感的突变体。在表达 gfp 的猪双歧杆菌中，ClpA 而不是 ClpB 参与了 42 ℃ 下绿色荧光蛋白的降解。我们得出的结论是，ClpB 负责耐受多种胁迫，并且恶劣环境条件引起的致死性可能具有相似的分子起源。
Pathogens often encounter stressful conditions inside their hosts. In the attempt to characterize the stress response in Brucella suis, a gene highly homologous to Escherichia coli clpB was isolated from Brucella suis, and the deduced amino acid sequence showed features typical of the ClpB ATPase family of stress response proteins. Under high-temperature stress conditions, ClpB of B. suis was induced, and an isogenic B. suis clpB mutant showed increased sensitivity to high temperature, but also to ethanol stress and acid pH. The effects were reversible by complementation. Simultaneous inactivation of clpA and clpB resulted in a mutant that was sensitive to oxidative stress. In B. suis expressing gfp, ClpA but not ClpB participated in degradation of the green fluorescent protein at 42 degreesC. We concluded that ClpB was responsible for tolerance to several stresses and that the lethality caused by harsh environmental conditions may have similar molecular origins.