Diversity of bacterial endosymbionts and bacteria-host co-evolution in Gondwanan relict moss bugs (Hemiptera: Coleorrhyncha: Peloridiidae)
Diversity of bacterial endosymbionts and bacteria-host co-evolution in Gondwanan relict moss bugs (Hemiptera: Coleorrhyncha: Peloridiidae)
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DOI：
10.1111/1462-2920.12101
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发表时间：
2013-07-01
影响因子：
5.1
通讯作者：
Hartung, Viktor
中科院分区：
文献类型：
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作者：
Kuechler, Stefan Martin;Gibbs, George;Hartung, Viktor
许多半翅目动物与共生细菌有关，共生细菌通常存在于特定的细菌体中。在这项研究中，我们首次对冈德南残存昆虫分类群中与细菌组相关的专性内共生体进行了分子鉴定，苔藓虫(半翅目：鞘翅目：斑潜蝇科)代表了半翅目中最古老的谱系之一。分析了来自南美洲、澳大利亚/塔斯马尼亚和新西兰的15种植物的内共生联系。基于4个16S-23S rRNA基因片段的系统发育分析表明，Peloridiidae专性内共生菌构成了一个未知的伽马保护性细菌群，在这里命名为Candidatus Evansia muelleri‘。它们与丝状内共生菌Candidatus Portiera和Candidatus Carsonella有亲缘关系。初级内生共生菌与寄主(COI+28S rRNA)树的比较表明，两者总体上是一致的，表明寄主及其共生体是共物种。以鱼类为研究对象，研究了内共生菌在昆虫体内的分布及其传播。在一对细菌体和每个发育卵母细胞后极的共生球中均可检测到内共生菌。此外，对马氏管的超微结构分析表明，大多数宿主核受到一种内共生的核内细菌的感染，该细菌被确定为立克次体属的一种甲型蛋白细菌。
Many hemipterans are associated with symbiotic bacteria, which are usually found intracellularly in specific bacteriomes. In this study, we provide the first molecular identification of the bacteriome-associated, obligate endosymbiont in a Gondwanan relict insect taxon, the moss bugs (Hemiptera: Coleorrhyncha: Peloridiidae), which represents one of the oldest lineages within the Hemiptera. Endosymbiotic associations of fifteen species of the family were analysed, covering representatives from South America, Australia/Tasmania and New Zealand. Phylogenetic analysis based on four kilobases of 16S-23S rRNA gene fragments showed that the obligate endosymbiont of Peloridiidae constitute a so far unknown group of Gammaproteobacteria which is named here Candidatus Evansia muelleri'. They are related to the sternorrhynchous endosymbionts CandidatusPortiera and CandidatusCarsonella. Comparison of the primary-endosymbiont and host (COI+28S rRNA) trees showed overall congruence indicating co-speciation the hosts and their symbionts. The distribution of the endosymbiont within the insect body and its transmission was studied using FISH. The endosymbionts were detected endocellularly in a pair of bacteriomes as well as in the symbiont ball' of the posterior pole of each developing oocyte. Furthermore, ultrastructural analysis of the Malpighian tubules revealed that most host nuclei are infected by an endosymbiotic, intranuclear bacterium that was determined as an Alphaproteobacterium of the genus Rickettsia.