Tobacco Mosaic Virus with Peroxidase-Like Activity for Cancer Cell Detection through Colorimetric Assay.
Tobacco Mosaic Virus with Peroxidase-Like Activity for Cancer Cell Detection through Colorimetric Assay.
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DOI：
10.1021/acs.molpharmaceut.7b00921
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发表时间：
2018-01
影响因子：
4.9
通讯作者：
Jiawang Guo;Xia Zhao;Jun Hu;Yuan Lin;Qian Wang
中科院分区：
文献类型：
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作者：
Jiawang Guo;Xia Zhao;Jun Hu;Yuan Lin;Qian Wang
基于细胞的酶联免疫吸附试验（CELLISA）因其简单、成本低而广泛应用于疾病诊断。最近，类过氧化物酶纳米材料已成为 CELLISA 应用的有前途的系统。本工作将烟草花叶病毒（TMV）与过氧化物酶类无机纳米颗粒（铂纳米颗粒）和癌细胞目标基团（叶酸，FA）同时定制，获得用于癌细胞检测的TMV-FA-Pt纳米颗粒。由于TMV颗粒均匀分布的反应基团和明确的结构，铂纳米颗粒可以在TMV的外表面上原位生长，具有优异的单分散性和均匀的空间分布。同时，通过 Cu(I) 催化的炔叠氮环加成反应（一种有效的“点击”化学），带有 PEG1000 连接体的 FA 成功与 TMV 的外壳蛋白缀合。我们的研究表明，所得的TMV-FA-Pt对癌细胞具有特异性亲和力，并成功用于通过CELLISA检测癌细胞。低于 1.0 × 104 个细胞/mL 的癌细胞可以轻松检测到。
Cell-based ELISA (CELLISA) has been widely used in disease diagnosis due to its simplicity and low cost. Recently, peroxidase-like nanomaterials have emerged as promising systems for CELLISA applications. In this work, tobacco mosaic virus (TMV) was simultaneously tailored with peroxidase-like inorganic nanoparticles (platinum nanoparticles) and cancer cell target groups (folic acid, FA) to obtain TMV-FA-Pt nanoparticles for cancer cell detection. Induced by the uniformly distributed reactive groups and well-defined structure of the TMV particle, platinum nanoparticles could be grown in situ on the exterior surface of TMV with excellent monodispersity and uniform spatial distribution. Meanwhile, FA with a PEG1000 linker was successfully conjugated to the coat proteins of TMV through the Cu(I)-catalyzed alkyne-azide cycloaddition reaction, an efficient "click" chemistry. Our study demonstrated that the resultant TMV-FA-Pt had specific affinity to cancer cells and was successfully used to detect cancer cells through CELLISA. Less than 1.0 × 104 cells/mL of cancer cells could be readily detected.