Antibacterial Effects of Green Tea Polyphenols on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus
Antibacterial Effects of Green Tea Polyphenols on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus
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DOI：
10.1007/s00284-008-9239-0
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发表时间：
2008-12-01
影响因子：
2.6
通讯作者：
Oh, Kye-Heon
中科院分区：
文献类型：
--
作者：
Cho, Yun-Seok;Schiller, Neal L.;Oh, Kye-Heon
评价了从韩国绿色茶（Camellia sinensis）中提取的茶多酚（TPP）对耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株的抗菌作用。苯唑西林对30 S的最小抑菌浓度（MIC）的表征。从用苯唑西林治疗的患者中分离的金黄色葡萄球菌菌株鉴定出13个苯唑西林MIC为4 μ g/mL的菌株为耐甲氧西林金黄色葡萄球菌（MRSA）（范围：8 - 512 μ g/mL），而17个菌株为甲氧西林敏感金黄色葡萄球菌（MSSA）（范围：0.25-0.5 μ g/mL）。TPP对MSSA和MRSA菌株的MIC范围为50至180 μ g/mL。当这些菌株与亚MIC（0.5x MIC）水平的TPP共孵育时，苯唑西林对于13种MRSA菌株中的每一种的MIC降低8倍至128倍，表明TPP加苯唑西林的组合对于所有临床MRSA分离株是协同的。双向聚丙烯酰胺凝胶电泳结果显示，TPP诱导MRSA-13细胞外14种蛋白表达下调，3种蛋白表达上调。这些研究表明，TPP可以差异刺激这些细菌中各种蛋白质的表达，并协同苯唑西林对MRSA的杀菌活性。
The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patients treated with oxacillin identified 13 strains with an oxacillin MIC 4 mu g/mL as methicillin-resistant Staphylococcus aureus (MRSA) (range: 8 to 512 mu g/mL), while 17 strains were methicillin-susceptible Staphylococcus aureus (MSSA) (range: 0.25-0.5 mu g/mL). The MICs of TPP ranged from 50 to 180 mu g/mL for both the MSSA and the MRSA strains. The MICs of oxacillin for each of the 13 MRSA strains were reduced between 8- and 128-fold when these strains were coincubated with sub-MIC (0.5x MIC) levels of TPP, demonstrating that the combination of TPP plus oxacillin was synergistic for all of the clinical MRSA isolates. Two-dimensional polyacrylamide gel electrophoresis identified 14 extracellular proteins of MRSA-13 down-regulated and 3 proteins up-regulated by exposure to TPP. These studies demonstrate that TPP can differentially stimulate the expression of various proteins in these bacteria and synergize the bactericidal activity of oxacillin for MRSA.