JAM-C is an apical surface marker for neural stem cells.
JAM-C is an apical surface marker for neural stem cells.
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DOI：
10.1089/scd.2011.0274
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发表时间：
2012-03
影响因子：
4
通讯作者：
Sandra Stelzer;M. M. Worlitzer-M.;L. Bahnassawy;K. Hemmer;Kirité Rugani;Inga Werthschulte;Anna-Lena Schön;Benjamin F. Brinkmann;E. Bunk;T. Palm;K. Ebnet;J. Schwamborn
中科院分区：
文献类型：
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作者：
Sandra Stelzer;M. M. Worlitzer-M.;L. Bahnassawy;K. Hemmer;Kirité Rugani;Inga Werthschulte;Anna-Lena Schön;Benjamin F. Brinkmann;E. Bunk;T. Palm;K. Ebnet;J. Schwamborn
交界性黏附分子-C(JAM-C)是一种表达于多种细胞类型的黏附性细胞表面蛋白。JAM-C定位于接触内皮细胞和上皮细胞之间的顶端局部紧密连接(TJ)，在那里它有助于细胞与细胞的粘连。就像那些上皮细胞一样，神经干细胞也是沿着它们的顶端-基底轴高度极化的。所有干细胞，包括神经干细胞(NSCs)的定义特征是它们自我更新的能力。这种自我更新依赖于对对称和不对称细胞分裂的严格控制。在神经干细胞中，分裂是对称的还是不对称的在很大程度上取决于顶膜的分布和细胞在细胞基极上的命运决定因素。在这项研究中，我们证明了JAM-C在胚胎和成年小鼠脑中的神经前体细胞和神经干细胞上表达。此外，我们证明在体内JAM-C在顶端表面表现出丰富，因此在细胞分裂期间是不对称分布的。这些结果将JAM-C定义为神经干细胞的一种新的表面标记。
Junctional adhesion molecule-C (JAM-C) is an adhesive cell surface protein expressed in various cell types. JAM-C localizes to the apically localized tight junctions (TJs) between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesions. Just as those epithelial cells, also neural stem cells are highly polarized along their apical-basal axis. The defining feature of all stem cells, including neural stem cells (NSCs) is their ability to self renew. This self-renewal depends on the tight control of symmetric and asymmetric cell divisions. In NSCs, the decision whether a division is symmetric or asymmetric largely depends on the distribution of the apical membrane and cell fate determinants on the basal pole of the cell. In this study we demonstrate that JAM-C is expressed on neural progenitor cells and neural stem cells in the embryonic as well as the adult mouse brain. Furthermore, we demonstrate that in vivo JAM-C shows enrichment at the apical surface and therefore is asymmetrically distributed during cell divisions. These results define JAM-C as a novel surface marker for neural stem cells.