Backscatter multiple wavelength digital holography for color micro-particle imaging
Backscatter multiple wavelength digital holography for color micro-particle imaging
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DOI：
10.1364/ao.441509
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发表时间：
2022-02-10
期刊：
影响因子：
1.9
通讯作者：
Berg, Matthew J.
中科院分区：
文献类型：
--
作者：
Giri, Ramesh;Berg, Matthew J.
这项工作适用于数字全息图像的彩色静止微粒。该方法涉及迈克尔逊干涉仪混合参考光与从粒子分布反向散射的弱强度光。为了实现彩色图像，使用三个波长430、532和633 nm作为主要光源。同时记录三个单独的后向散射全息图，每个波长一个，使用三CMOS棱镜传感器在没有光谱串扰的情况下解决。菲涅耳衍射理论用于从每个全息图绘制单色图像。然后，通过以红色、绿色和蓝色作为原色的加色混合来组合图像。其结果是一个彩色图像类似的外观与传统的显微镜在白光落射照明模式。各种彩色聚乙烯微球和非球形尘埃粒子的实验结果表明了该方法的可行性，并说明了简单的斑点噪声抑制和白色平衡方法的效果。最后，应用能够以定量和客观的方式区分不同颜色的颗粒的色度分析。(C)根据OSA开放获取出版协议的条款，2021年美国光学学会
This work applies digital holography to image stationary micro-particles in color. The approach involves a Michelson interferometer to mix reference light with the weak intensity light backscattered from a distribution of particles. To enable color images, three wavelengths are used, 430, 532, and 633 nm, as primary light sources. Three separate backscattered holograms are recorded simultaneously, one for each wavelength, which are resolved with-out spectral cross talk using a three-CMOS prism sensor. Fresnel diffraction theory is used to render monochrome images from each hologram. The images are then combined via additive color mixing with red, green, and blue as the primary colors. The result is a color image similar in appearance to that obtained with a conventional microscope in white-light epi-illumination mode. A variety of colored polyethylene micro-spheres and nonspherical dust particles demonstrate the feasibility of the approach and illustrate the effect of simple speckle-noise suppression and white balance methods. Finally, a chromaticity analysis is applied that is capable of differentiating particles of different colors in a quantitative and objective manner. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement