The High Osmotic Response and Cell Wall Integrity Pathways Cooperate to Regulate Transcriptional Responses to Zymolyase-induced Cell Wall Stress in Saccharomyces cerevisiae
The High Osmotic Response and Cell Wall Integrity Pathways Cooperate to Regulate Transcriptional Responses to Zymolyase-induced Cell Wall Stress in Saccharomyces cerevisiae
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DOI：
10.1074/jbc.m808693200
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发表时间：
2009-04-17
影响因子：
4.8
通讯作者：
Arroyo, Javier
中科院分区：
文献类型：
--
作者：
Garcia, Raul;Rodriguez-Pena, Jose M.;Arroyo, Javier
酿酒酵母对细胞壁完整性严重受损的适应主要涉及细胞壁完整性(CWI)途径。然而，在最近的工作(Bermejo，C.，Rodriguez，E.，Garcia，R.，Rodriguez-Pena，J.M.，Rodriguez de la Concepcion，M.L.，Rivas，C.，Arias，P.，Nombela，C.，Posas，F.和Arroyo，J.(2008)Mol.比奥尔。细胞19,1113-1124)，我们已经证明了高渗透压反应(HOG)途径的共同参与，以确保酵母在发酵酶介导的细胞壁压力下存活，酵母酶可以降解β-1，3葡聚糖网络。在这里，我们利用全基因组表达谱表征了这两条通路在酵母对酵解酶处理的整体转录反应调节中的作用。酵母菌的一个主要基因群依赖于MAPKs SLT2和Hog1的诱导。这些基因的转录激活依赖于MAPKKK Bck 1、转录因子Rlm1和HOG途径Sho1分支的元件，而不依赖于Cwi途径的传感器。第二组基因依赖于SLT2，而不是Hog1或PBS2。然而，这些基因的诱导依赖于HOG途径的上游元件，如Sho1、Ste50和Ste11，这与HOG和CWI途径的顺序激活一致。发酵酶还通过激活一组依赖于HOG途径Sho1分支的元件而不依赖于SLT2的基因来促进渗透性转录反应，其中许多基因的诱导依赖于MSN2/4。此外，在没有Hog1的情况下，由于这些途径与HOG途径之间的相互作用，发酵酶诱导了与交配和丝化相关的另一种反应。最后，在没有SLT2的情况下，发酵酶增加了与野生型渗透适应相关的基因的诱导，这表明CWI途径对HOG途径具有抑制作用。这些研究清楚地揭示了负责调节酵母对细胞壁压力的适应反应的信号转导机制的复杂性。
The adaptation of Saccharomyces cerevisiae to situations in which cell wall integrity is seriously compromised mainly involves the cell wall integrity (CWI) pathway. However, in a recent work (Bermejo, C., Rodriguez, E., Garcia, R., Rodriguez-Pena, J. M., Rodriguez de la Concepcion, M. L., Rivas, C., Arias, P., Nombela, C., Posas, F., and Arroyo, J. (2008) Mol. Biol. Cell 19, 1113-1124) we have demonstrated the co-participation of the high osmotic response (HOG) pathway to ensure yeast survival to cell wall stress mediated by zymolyase, which hydrolyzes the beta-1,3 glucan network. Here we have characterized the role of both pathways in the regulation of the overall yeast transcriptional responses to zymolyase treatment using whole genome expression profiling. A main group of yeast genes is dependent on both MAPKs, Slt2 and Hog1, for their induction. The transcriptional activation of these genes depends on the MAPKKK Bck1, the transcription factor Rlm1, and elements of the sho1 branch of the HOG pathway, but not on the sensors of the CWI pathway. A second group of genes is dependent on Slt2 but not Hog1 or Pbs2. However, the induction of these genes is dependent on upstream elements of the HOG pathway such as Sho1, Ste50, and Ste11, in accordance with a sequential activation of the HOG and CWI pathways. Zymolyase also promotes an osmotic-like transcriptional response with the activation of a group of genes dependent on elements of the Sho1 branch of HOG pathway but not on Slt2, with the induction of many of them dependent on Msn2/4. Additionally, in the absence of Hog1, zymolyase induces an alternative response related to mating and filamentation as a consequence of the cross-talk between these pathways and the HOG pathway. Finally, in the absence of Slt2, zymolyase increases the induction of genes associated with osmotic adaptation with respect to the wild type, suggesting an inhibitory effect of the CWI pathway over the HOG pathway. These studies clearly reveal the complexity of the signal transduction machinery responsible for regulating yeast adaptation responses to cell wall stress.