Mapping of retinal projections in the living rat using high-resolution 3D gradient-echo MRI with Mn2+-induced contrast
Mapping of retinal projections in the living rat using high-resolution 3D gradient-echo MRI with Mn2+-induced contrast
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DOI：
10.1002/mrm.1209
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发表时间：
2001-09-01
影响因子：
3.3
通讯作者：
Frahm, J
中科院分区：
文献类型：
--
作者：
Watanabe, T;Michaelis, T;Frahm, J
本研究使用高分辨率t- 1加权3D梯度回声MRI （195 x 195 x 125 mum(3)）描述了活体大鼠在眼内注射Mn2+ （0.1 μ l 1m MnCl2水溶液）后8、24、48和72小时的视觉通路的神经轴突追踪。注射后24小时获得最佳结果，显示从视网膜、视神经和交叉到对侧视束、膝状外侧核背侧和腹侧、上丘及其臂、橄榄前盖核、视束核和视交叉上核呈连续的顺行标记模式。这些结果强调了单次注射Mn2+后在活体动物中重复MRI通道追踪的可行性。该方法有望推进行为动物神经轴突功能的研究，特别强调在发育神经生物学中的应用。(C) 2001 Wiley-Liss, Inc。
This study describes the neuroaxonal tracing of the visual pathway in the living rat using high-resolution T-1-weighted 3D gradient-echo MRI (195 x 195 x 125 mum(3)) at 8, 24, 48, and 72 h after intraocular Mn2+ injection (0.1 mul of 1 M aqueous MnCl2). Best results were obtained at 24 h postinjection, revealing a continuous pattern of anterograde labeling from the retina, optic nerve, and chiasm to the contralateral optic tract, the dorsal and ventral lateral geniculate nucleus, the superior colliculus and its brachium, the olivary pretectal nucleus, the nucleus of the optic tract, and the suprachiasmatic nucleus. These results underline the feasibility of repeated MRI tract tracing in living animals after a single injection of Mn2+. The approach is expected to advance studies of neuroaxonal function in behaving animals with special emphasis on applications in developmental neurobiology. (C) 2001 Wiley-Liss, Inc.