Optimization of total flavonoids content extracted from Flos Populi using response surface methodology
Optimization of total flavonoids content extracted from Flos Populi using response surface methodology
复制标题
DOI：
10.1016/j.indcrop.2012.08.020
复制
发表时间：
2013-05-01
影响因子：
5.9
通讯作者：
Li, Yan-Hua
中科院分区：
文献类型：
--
作者：
Sheng, Zun-Lai;Wan, Peng-Fei;Li, Yan-Hua
杨树花是一种优良的总黄酮类化合物来源，可用作抗真菌药物。为了更好地利用这一资源，采用Box-Behnken试验设计对杨杨花总黄酮的提取工艺进行优化。考察了提取时间、提取温度、乙醇浓度和料液比4个因素对黄酮提取率的影响，并采用响应面法对黄酮提取的最佳工艺条件进行了优化。数学回归模型的相关性分析表明，二次多项式模型可用于描述总黄酮的提取工艺。响应面分析表明，这些自变量对总黄酮提取率有显著影响。结果表明，在温度94.66 ℃、乙醇浓度45.92%、液料比18.55：1（mL/g）、提取时间2.27 h的条件下，总黄酮提取率最高。在最佳工艺条件下，总黄酮的平均得率为53.08 ± 0.43 mg/g，与预测值52.04 mg/g基本一致。该提取方法成功地应用于杨树花中总黄酮的提取。(C)2012爱思唯尔有限公司版权所有。
Flos Populi is an excellent source of total flavonoids used as an antidiarrheal drug. For better utilization of the resource, Box-Behnken design was used to optimize the extraction conditions of total flavonoids from Flos Populi. The effects of four independent variables in terms of extraction time, temperature, ethanol concentration, and the ratio of liquid to material on the flavonoids yield were determined and the optimal conditions for flavonoids were evaluated by means of response surface methodology. Correlation analysis of the mathematical regression model indicated that a quadratic polynomial model could be employed to characterize extraction process for the total flavonoids. Response surface plots showed that these independent variables significantly influenced the extraction yield of total flavonoids. The optimal extraction parameters to obtain the highest total flavonoids yield were temperature of 94.66 degrees C, ethanol concentration of 45.92%, the ratio of liquid to material of 18.55:1 (mL/g), and time duration of 2.27 h. The average experimental total flavonoids yield under the optimum conditions was found to be 53.08 +/- 0.43 mg/g, which agreed with the predicted value of 52.04 mg/g. The extraction method was applied successfully to extract total flavonoids from Flos Populi. (C) 2012 Elsevier B.V. All rights reserved.