Host-Guest Interactions in Fe(III)-Trimesate MOF Nanoparticles Loaded with Doxorubicin
Host-Guest Interactions in Fe(III)-Trimesate MOF Nanoparticles Loaded with Doxorubicin
复制标题
DOI：
10.1021/jp503809w
复制
发表时间：
2014-07-24
影响因子：
3.3
通讯作者：
Monti, Sandra
中科院分区：
文献类型：
--
作者：
Anand, Resmi;Borghi, Francesco;Monti, Sandra
在中性Tris缓冲液中，通过紫外-可见吸收光谱、圆二色光谱和荧光光谱研究了多柔比星（DOX）在Fe（III）-trimesate金属有机骨架（MIL-100（Fe））多孔纳米粒子中的包封.通过吸收和荧光滴定法测定了DOX-MIL-100（Fe）复合物的结合常数和绝对光谱。DOX以1：1的化学计量比与脱水的Fe_3 O（OH）（H_2 O）（2）[（C_6 H_3）（CO_2）（3）]（2）结构单元结合，表观结合常数为（1.1 ~ 1.8）× 10（4）M ~（-1）。光谱数据表明，DOX结合通过糖苷配基部分的一个或两个去质子化羟基基团和配位不饱和Fe（III）中心之间形成高度稳定的配位键而发生。DOX的荧光完全淬灭和显着的热和光化学稳定性观察到DOX纳入MIL-100（Fe）框架。
Doxorubicin (DOX) entrapment in porous Fe(III)-trimesate metal organic frameworks (MIL-100(Fe)) nanoparticles was investigated in neutral Tris buffer via UV-vis absorption, circular dichroism (CD), and fluorescence. The binding constants and the absolute spectra of the DOX-MIL-100(Fe) complexes were determined via absorption and fluorescence titrations. A binding model where DOX associates as monomer to the dehydrated Fe3O (OH)(H2O)(2) [(C6H3)(CO2)(3)](2) structural unit in 1:1 stoichiometry, with apparent association constant of (1.1 to 1.8) x 10(4) M-1, was found to reasonably fit the experimental data. Spectroscopic data indicate that DOX binding occurs via the formation of highly stable coordination bonds between one or both deprotonated hydroxyl groups of the aglycone moiety and coordinatively unsaturated Fe(III) centers. Complete quenching of the DOX fluorescence and remarkable thermal and photochemical stability were observed for DOX incorporated in the MIL-100(Fe) framework.