Tectorigenin reduces type IV pilus-dependent cell adherence in Clostridium perfringens
Tectorigenin reduces type IV pilus-dependent cell adherence in Clostridium perfringens
复制标题
鸢尾皂甙元可降低产气荚膜梭菌中 IV 型菌毛依赖性细胞粘附
DOI：
10.1093/femsle/fnz112
复制
发表时间：
2019-05-01
影响因子：
2.1
通讯作者：
Deng, Xuming
中科院分区：
文献类型：
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作者：
Liu, Shui;Wang, Jianfeng;Deng, Xuming
产气荚膜梭菌是一种厌氧的革兰氏阳性细菌，在地球仪周围的人类和动物中引起一系列疾病。IV型菌毛（TFP）系统在宿主细胞的定殖和侵袭、生物被膜的形成和滑行运动中起着关键作用，对C.产气荚膜杆菌感染因此，靶向TFP功能可能是治疗C.产气荚膜杆菌感染本文研究了从中药射干（Belamcanda chinensis（L.）DC对C.产气荚膜杆菌鸢尾苷元显著抑制Caco-2细胞的滑动运动、生物膜形成和粘附，而对C.产气荚膜杆菌此外，我们还证明了TE对TFP功能的抑制作用似乎部分通过抑制TFP相关基因来实现。这些结果表明，TE可能被开发为一种新的抗C.产气荚膜杆菌感染，特别是用于靶向TFP。
Clostridium perfringens is an anaerobic, Gram-positive bacterium that causes a range of diseases in humans and animals around the globe. The type IV pilus (TFP) system plays a key role in the colonization and invasion of host cells, biofilm formation and gliding motility, which is vital for C. perfringens infection. Therefore, targeting TFP function may be a promising strategy for the treatment of C. perfringens infection. Here, we investigated the potential inhibitory effects of tectorigenin (TE), an isoflavone extracted from the rhizome of the Chinese herb Belamcanda chinensis (L.) DC, on gliding motility, biofilm formation, adherence to cells and antibacterial activity of C. perfringens. Tectorigenin significantly inhibited gliding motility, biofilm formation and adherence to Caco-2 cells without observable antibacterial activity against C. perfringens. In addition, we also demonstrated that the inhibitory effect of TE on TFP function appears to be partially achieved by the suppression of TFP-associated genes. These findings demonstrate that TE may have the potential to be developed as a new anti-virulence drug for C. perfringens infection, particularly for the targeting of TFP.