The effect of protease inhibitors, leupeptin and E64d, on differentiation of C2C12 myoblasts in tissue culture.
The effect of protease inhibitors, leupeptin and E64d, on differentiation of C2C12 myoblasts in tissue culture.
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蛋白酶抑制剂亮肽素和 E64d 对组织培养中 C2C12 成肌细胞分化的影响。
DOI：
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发表时间：
1992
影响因子：
1.6
通讯作者：
A. Stracher
中科院分区：
文献类型：
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作者：
A. Kumar;S. Shafiq;R. Wadgaonkar;A. Stracher
细胞内钙水平在肌原纤维分解和肌纤维再生中起重要作用。早期的研究表明，钙活化蛋白酶（calpain）参与了横纹肌肌原纤维中z盘的去除，而三肽醛（calpain的抑制剂）白细胞介素（lepeptin）抑制了这一活性。在本研究中，我们证明了胰肽和另一种钙蛋白酶抑制剂E64d在组织培养中抑制小鼠骨骼肌C2C12成肌细胞融合形成多核肌管。
Intracellular calcium levels play an important role in myofibril disintegration and regeneration of muscle fibers. Earlier studies have shown that the calcium activated protease, calpain, is involved in the removal of Z-discs from myofibrils of striated muscle and the tripeptide-aldehyde, leupeptin, which is an inhibitor of calpain, inhibits this activity. In the present communication, we demonstrate that leupeptin and another calpain inhibitor, E64d, inhibit the fusion of mouse skeletal muscle C2C12 myoblasts to form multinucleated myotubes in tissue culture.