Interaction of Aspergillus with human respiratory mucosa: a study with organ culture model
Interaction of Aspergillus with human respiratory mucosa: a study with organ culture model
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DOI：
10.1080/13693780802558959
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发表时间：
2009-01-01
期刊：
影响因子：
2.9
通讯作者：
Kawanami, R.
中科院分区：
文献类型：
--
作者：
Amitani, R.;Kawanami, R.
本文研究了烟曲霉菌在器官培养模型中与人支气管粘膜的相互作用。A.将烟曲霉分生孢子接种到器官培养组织上并孵育24小时。在各时间点（1、6、12、18和24 h），测定支气管上皮细胞纤毛搏动频率（CBF），观察A.烟曲霉分生孢子（和菌丝），以及上皮结构的变化，通过扫描和透射电子显微镜进行了研究。本研究表明，A.烟曲霉对支气管上皮造成损害，这与CBF减慢有关。此外，研究还表明，A.烟曲霉的菌丝被内化在纤毛和非纤毛上皮细胞内，一些分生孢子位于上皮的细胞间隙内，并且菌丝穿透上皮的细胞间隙和细胞内间隙。这些发现表明曲霉菌侵入支气管粘膜可能至少有三种途径：（1）菌丝穿透上皮细胞间隙;（2）菌丝直接穿透上皮细胞;（3）分生孢子在上皮细胞内内化。
The interaction of Aspergillus fumigatus with human bronchial mucosa in an organ culture model, with an air-mucosal interface, was studied. A. fumigatus conidia were inoculated onto the organ culture tissues and incubated for 24 h. At each time point (1, 6, 12, 18 and 24 h), after the measurement of ciliary beat frequency (CBF) of bronchial epithelial cells, adherence and invasion of the epithelium by A. fumigatus conidia (and hyphae), as well as structural changes of the epithelium, were investigated by scanning and transmission electron microscopy. This study demonstrated that A. fumigatus caused damage to the bronchial epithelium that was associated with CBF slowing. In addition, the study demonstrated that some of the conidia of A. fumigatus were internalized within ciliated and non-ciliated epithelial cells, some conidia were located within the intercellular spaces of the epithelium, and that hyphae penetrated through both intercellular and intracellular spaces of the epithelium. These findings suggest there might be at least three pathways by which Aspergillus invades the bronchial mucosa: (1) penetration of hyphae through the intercellular spaces in the epithelium; (2) direct penetration of hyphae through epithelial cells; and (3) internalization of conidia within epithelial cells.