The GAR domain of GAS2L3 mediates binding to the chromosomal passenger complex and is required for localization of GAS2L3 to the constriction zone during abscission
The GAR domain of GAS2L3 mediates binding to the chromosomal passenger complex and is required for localization of GAS2L3 to the constriction zone during abscission
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DOI：
10.1111/febs.12766
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发表时间：
2014-05-01
期刊：
影响因子：
5.4
通讯作者：
Gaubatz, Stefan
中科院分区：
文献类型：
--
作者：
Fackler, Marc;Wolter, Patrick;Gaubatz, Stefan
GAS 2L 3是最近鉴定的一种微管蛋白和肌动蛋白结合蛋白，其调节胞质分裂和胞质分裂。在这项研究中，我们发现GAS 2L 3与染色体乘客复合物（CPC）相互作用，在有丝分裂和胞质分裂中起着关键作用。生化分析表明，GAS 2L 3直接与borealin的C-末端和生存素的N-末端相互作用。我们发现这两个CPC亚基与GAS 2L 3之间的相互作用是由GAS 2L 3的保守GAR结构域介导的。我们进一步表明，GAS 2L 3的GAR结构域是必需的GAS 2L 3的收缩区的本地化。总之，这些数据表明，GAS 2L 3是在细胞动力学终止过程中CPC的下游效应子。
GAS2L3 is a recently identified tubulin- and actin-binding protein that regulates cytokinesis and abscission. In this study we show that GAS2L3 interacts with the chromosomal passenger complex (CPC), which plays key roles in mitosis and cytokinesis. Biochemical assays show that GAS2L3 directly interacts with the C-terminus of borealin and the N-terminus of survivin. We find that the interaction between these two CPC subunits and GAS2L3 is mediated by the conserved GAR domain of GAS2L3. We further show that the GAR domain of GAS2L3 is required for localization of GAS2L3 to the constriction zone. Taken together these data suggest that GAS2L3 is a downstream effector of the CPC during cytokinetic abscission.