Herpes simplex virus glycoprotein D is sufficient to induce spontaneous pH-independent fusion in a cell line that constitutively expresses the glycoprotein.
Herpes simplex virus glycoprotein D is sufficient to induce spontaneous pH-independent fusion in a cell line that constitutively expresses the glycoprotein.
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单纯疱疹病毒糖蛋白 D 足以在组成型表达该糖蛋白的细胞系中诱导自发的不依赖于 pH 的融合。
DOI：
10.1016/0042-6822(88)90533-8
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Roizman,B
中科院分区：
文献类型：
--
作者：
Campadelli-Fiume,G;Avitabile,E;Fini,S;Stirpe,D;Arsenakis,M;Roizman,B
在一个命名为BJ-0的细胞系中检测到自发性小多核细胞，该细胞系含有单纯疱疹病毒1型DNA的BamHI J片段，并组成性表达糖蛋白D（gD）。BJ-0细胞的融合活性与gD的产生相关，并且在细胞暴露于针对gD的单克隆抗体HD 1后急剧降低。对依赖于gD的细胞融合的特征和要求的研究得出结论，即BJ-0细胞的gD介导的融合活性的特征和要求与先前报道的由病毒诱导的细胞融合的特征和要求相似，因为（i）多核细胞增多症不因暴露于低pH培养基而增加，有或没有预先暴露于胰蛋白酶，（ii）用神经氨酸酶去除末端唾液酸残基后，多核细胞的数量减少;（iii）用伴刀豆球蛋白A或其还原形式琥珀酰伴刀豆球蛋白A掩蔽高甘露糖N-连接寡糖，多核细胞的数量增加。这种效应被与人鼻子的竞争所逆转。
Spontaneous small polykaryocytes were detected in a cell line designated BJ-o that harbors theBamHI J fragment of herpes simplex virus 1 DNA and expresses constitutively glycoprotein D (gD). The fusion activity of BJ-o cells correlated with gD production and was drastically reduced following exposure of the cells to monoclonal antibody HD1 to gD. Studies on the characteristics and requirements of cell fusion dependent on gD led to the conclusion that the characteristics and requirements for gD-mediated fusion activity of BJ-o cells are similar to those previously reported for cell fusion induced by the virus in that (i) polykaryocytosis was not augmented by exposure to medium of low pH with or without prior exposure to trypsin, (ii) the number of polykaryocytes was reduced following removal of terminal sialic acid residues by neuraminidase, and (iii) the number of polykaryocytes was augmented by masking of high-mannose N-linked oligosaccharides with concanavalin A or with its reduced form, succinyl concanavalin A. This effect was reversed by competition with man nose.