[Acupoint sensitization enhances inhibitory effect of electroacupuncture on jejunum mobility in rats].
[Acupoint sensitization enhances inhibitory effect of electroacupuncture on jejunum mobility in rats].
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DOI：
10.13702/j.1000-0607.200778
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发表时间：
2021-01-25
期刊：
影响因子：
--
通讯作者：
Zhu, Bing
中科院分区：
文献类型：
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作者：
Li, Wu;Xie, Xiao-Yin;Zhu, Bing
目的：观察针刺敏化与非敏化“天枢”穴（ST25）对大鼠空肠运动的效应差异。
方法：将雄性SD大鼠随机分为空白对照组、敏化ST25组（敏化组）和非敏化ST25组（非敏化组），每组16只。分别在左侧ST25区域注射15%芥子油（20 μL）和石蜡油（20 μL）诱导敏化和非敏化。通过记录舔舐局部敏化皮肤区域的次数和持续时间来评估大鼠的行为反应。实验结束时，经常规心脏灌注并用10%中性甲醛固定后，取ST25区域（1 cm×1 cm）的皮肤和肌肉组织进行H.E.染色，观察局部组织病理学变化。通过连接小气囊的压力传感器和放大器记录空肠收缩压力波来检测肠道运动，然后计算电针刺激前后收缩波幅度和频率的比值。在敏化和非敏化ST25区域施加电针（2 Hz，0.2 - 6.0 mA）20 s，比较其对空肠运动的影响。
结果：与空白对照组相比，芥子油注射而非石蜡油注射导致ST25区域组织出现组织学水肿和中性粒细胞浸润，舔舐次数和持续时间也显著增加（P<...此处原文似乎不完整）
OBJECTIVE: To observe the effect difference of electroacupuncture (EA) at the sensitized and non-sensitized acupoint "Tianshu" (ST25) on the motility of jejunum in rats.METHODS: Male SD rats were randomly divided into blank control, sensitized ST25 (sensitization), and non-sensitized ST25 (non-sensitization) groups (n=16 in each group). The sensitization and non-sensitization were induced by injection of 15% mustard oil (20 muL) and Paraffin oil (20 muL) into the left ST25 area respectively. The rats' behavior reactions were assessed by recording the numbers and duration of licking the local sensitized skin region. At the end of experiments and after regular trans-cardiac perfusion and fixing with 10% neutral formaldehyde, the skin and muscular tissues of the ST25 region (1 cm*1 cm) were taken for H.E. staining to observe the local histopathologic changes. The intestinal motility was detected by recording the contraction pressure waves of jejunum through a small balloon-connected pressure transducer and an amplifier, followed by calculating the ratios of amplitude and frequency of contraction waves between pre- and post-EA stimulation. EA (2 Hz, 0.2-6.0 mA) was applied to the sensitized and non-sensitized ST25 regions for 20 s for comparing their effects on jejunum motility.RESULTS: Compared with the blank control group, mustard oil injection but not Paraffin oil injection resulted in histological edema and neutrophils infiltration in tissues of ST25 region, and also striking increase of the number and duration of licking (P