The importance of transmission time in HIV infections and an epidemiological prospective follow-up study for 1 year in the Marmara Region of Turkey
The importance of transmission time in HIV infections and an epidemiological prospective follow-up study for 1 year in the Marmara Region of Turkey
复制标题
DOI：
10.1016/j.transci.2012.03.003
复制
发表时间：
2012-08-01
影响因子：
1.9
通讯作者：
Kocazeybek, Bekir
中科院分区：
文献类型：
--
作者：
Celik, Deniz Gozde;Yuksel, Pelin;Kocazeybek, Bekir
重要的是要发现最近和新的HIV/1感染，并采取预防措施，以防止艾滋病的快速疾病进展和降低感染率。我们的目的是通过确定首次检测到HIV/1血清阳性病例的传播时间来检测长期或近期的HIV感染。对323例经ELISA和Western-blotting检测为阳性的血清标本进行检测。通过Aware BED-EIA、HIV-1发病率检测（IgG捕获HIV-EIA）检测确定传播时间，区分长期和近期HIV/1感染。选择96名HIV抗体检测阴性且患有慢性传染病1年以上的健康献血员作为阴性对照组。在长期和最近的HIV/1感染的歧视，只有在体外ODn值。以市售试剂盒测定的标准化光密度（OD标本/OD校准品）< 0.8者为HIV近期感染者（155天或血清阳转<6个月）。ODn >1.2者为长期HIV/1感染者（病程> 155天或> 6个月）。ODn在0.8和1.2之间的病例被接受为“需要额外检测”病例。我们在323例病例中的263例（81.5%）中检测到最近的HIV/1感染（6个月）。在长期和近期HIV/1感染者中，最常见的传播途径是异性性交，分别为54例（50%）和257例（97%）。新感染者中已婚女性占63.3%，新近感染者中男性占65.3%，长期HIV/1感染者的检出率（81.5%）高于新近感染者（18.5%），提示长期HIV/1感染者可能有与HIV/1传播有关的活动。在异性性行为者和已婚男性中发现较高的艾滋病毒/1感染率，这表明这种情况对社会健康构成了极大的威胁。(C)2012爱思唯尔有限公司保留所有权利。
It is important to detect recent and new HIV/1 infections and to take preventative measures in order to prevent rapid disease progression in AIDS and to decrease the incidence of infection. We aimed to detect long standing or recent HIV infections by determining transmission times for the cases in which first-time HIV/1 seropositivity were detected. The serum samples of 323 cases which were found to be seropositive by ELISA and Western-blotting were included in this study. The discrimination between long-term and recent HIV/1 infection was made by determining transmission-time with the Aware BED-EIA, HIV-1 incidence test (IgG capture HIV-EIA) tests. Ninety-six healthy blood donors who did not have a positive anti-HIV test and a chronic infectious disease for at least 1 year were included in this study as a negative healthy control group. In the discrimination of long-term and recent HIV/1 infections, only in vitro ODn values were used. The cases with normalized optical density (OD) (ODspecimen/ODcalibrator) < 0.8 by commercial kit were accepted as recent HIV infection (155 days history or seroconversion less than 6 months). The cases with ODn >1.2 were accepted as long-term HIV/1 infections (more than 155 days history or more than 6 months). The cases with ODn between 0.8 and 1.2 were accepted as "additional tests needed" cases. We detected recent HIV/1 infections (6 months) in 263 (81.5%) out of 323 cases. The most frequently encountered transmission route in long-term and recent HIV/1 infections was heterosexual sexual intercourse as 54(50%) and 257 (97%), respectively. 63.3% of newly infected patients were married females and 65.3% of recently infected patients were males.In conclusion, the detection of the high ratio of long-term HIV/1 infection cases (81.5%) compared to recent infections (18.5%) suggested to us, that the long standing cases may have some activities related with transmission of HIV/1 in the past. The detection of higher HIV/1-infections in individuals which had heterosexual sex and also in married males suggested that this situation poses a very great threat for the health of society. (C) 2012 Elsevier Ltd. All rights reserved.