Profiling of gender-specific gene expression for Trichostrongylus vitrinus (Nematoda: Strongylida) by microarray analysis of expressed sequence tag libraries constructed by suppressive-subtractive hybridisation
Profiling of gender-specific gene expression for Trichostrongylus vitrinus (Nematoda: Strongylida) by microarray analysis of expressed sequence tag libraries constructed by suppressive-subtractive hybridisation
复制标题
DOI：
10.1016/j.ijpara.2003.12.007
复制
发表时间：
2004-04-01
影响因子：
4
通讯作者：
Gasser, RB
中科院分区：
文献类型：
--
作者：
Nisbet, AJ;Gasser, RB
Gencler特异性基因表达在Trichostrongylus vitrinus（strongylida）中通过使用抑制性提取的杂交方法来构建，男性和女性特异性基因档案，并从这些档案中对表达的序列标签进行测序与Caenorhabdisis Istis Is elembditis elembditis elembdisis elembdision archives进行测序通过微阵列和大型阵列分析，有机体和代表性子集的代表性子集的表达分析表达了序列标签。在这些T Vitrinus表达的序列标签中，有391个与秀丽隐杆线虫基因具有序列同源性。在其余表达的序列标签中，有62个与其他寄生虫种类的基因具有同源性，而263个表达的序列标签没有显着同源性。表达分析显示716 T玻璃体中561的性别特异性表达表达序列标签。在cDNA档案中，男性特异性蛋白激酶和蛋白质磷酸酶，参与碳水化合物代谢的主要精子蛋白和酶很丰富。雌性特异性卵黄蛋白，热震蛋白和伴侣蛋白也得到高度代表。参与许多细胞过程的基因，例如泛素化和蛋白酶体功能，基因转录，细胞信号传导。蛋白质 - 蛋白质相互作用和染色质组装和功能也以性别特异性表达。讨论了这些基因在寄生线虫中的配子发生，胚胎发生和繁殖中的潜在作用，该基因与其同源物在秀丽隐杆线虫中的已知作用有关。 （c）2004年澳大利亚寄生虫学会，由Elsevier Ltd.出版。保留所有权利。
Gencler-specific gene expression in Trichostrongylus vitrinus (order Strongylida) was investigated by constructing, male- and female-specific gene archives using a suppressive-subtractive hybridisation approach, sequencing of expressed sequence tags from these archives, comparison with genes of Caenorhabditis elegans and other organisms, and expression profiling of a representative subset of 7 16 expressed sequence tags by microarray and macroarray analysis. Of these T vitrinus expressed sequence tags, 391 had sequence homology to C. elegans genes. Of the remaining expressed sequence tags, 62 had homology to genes of other species of parasitic nematodes, and 263 expressed sequence tags had no significant homology. Expression profiling showed gender-specific expression for 561 of the 716 T vitrinus expressed sequence tags. Male-specific protein kinases and protein phosphatases, major sperm proteins and enzymes involved in carbohydrate metabolism were abundant in the cDNA archive. Female-specific vitellogenins, heat-shock proteins and chaperonins were also highly represented. Genes involved in a number of cellular processes, such as Ubiquitination and proteasome function, gene transcription, cell signalling. protein-protein interactions and chromatin assembly and function were also expressed in a gender-specific manner. The potential roles of these genes in gametogenesis, embryogenesis and reproduction in the parasitic nematode are discussed in relation to the known roles of their homologues in C. elegans. (C) 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.