Adaptive Use of Bubble Wrap for Storing Liquid Samples and Performing Analytical Assays
Adaptive Use of Bubble Wrap for Storing Liquid Samples and Performing Analytical Assays
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DOI：
10.1021/ac501206m
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发表时间：
2014-08-05
影响因子：
7.4
通讯作者：
Whitesides, George M.
中科院分区：
文献类型：
--
作者：
Bwambok, David K.;Christodouleas, Dionysios C.;Whitesides, George M.
本文表明，通常被称为“气泡包装”的包装材料中的充气隔间可以在资源有限的地区作为容器来储存液体样品，并进行生物分析。气泡包装的气泡很容易填充，通过使用带有针头或移液管尖端的注射器将样品注入其中，然后用指甲硬化剂密封孔。气泡在光谱的可见光范围内是透明的，可以用作吸光度和荧光测量的“比色皿”。这些气泡的内部是无菌的，并且允许在不需要昂贵的灭菌设备的情况下存储样品。气泡也是可渗透气体的，并且可以用于培养和储存微生物。通过结合碳电极，这些气泡可以用作电化学电池。本文通过培养大肠杆菌证明了气泡的能力。coli，生长C.用电化学方法测量亚铁氰化物，所有这些都在气泡内进行。
This paper demonstrates that the gas-filled compartments in the packing material commonly called "bubble wrap" can be repurposed in resource-limited regions as containers to store liquid samples, and to perform bioanalyses. The bubbles of bubble wrap are easily filled by injecting the samples into them using a syringe with a needle or a pipet tip, and then sealing the hole with nail hardener. The bubbles are transparent in the visible range of the spectrum, and can be used as "cuvettes" for absorbance and fluorescence measurements. The interiors of these bubbles are sterile and allow storage of samples without the need for expensive sterilization equipment. The bubbles are also permeable to gases, and can be used to culture and store micro-organisms. By incorporating carbon electrodes, these bubbles can be used as electrochemical cells. This paper demonstrates the capabilities of the bubbles by culturing E. coli, growing C. elegans, measuring glucose and hemoglobin spectrophotometrically, and measuring ferrocyanide electrochemically, all within the bubbles.