Correlation of visual in vitro cytotoxicity ratings of biomaterials with quantitative in vitro cell viability measurements
Correlation of visual in vitro cytotoxicity ratings of biomaterials with quantitative in vitro cell viability measurements
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DOI：
10.1007/s10565-007-9040-z
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发表时间：
2008-08-01
影响因子：
6.1
通讯作者：
Yetter, Ann B.
中科院分区：
文献类型：
--
作者：
Bhatia, Sujata K.;Yetter, Ann B.
通常使用细胞-材料相互作用的视觉评分来评估医疗器械和植入生物材料的生物反应性。在此类试验中，基于形态细胞变化（包括细胞溶解、变圆、扩散和增殖）的视觉证据，对生物材料进行细胞毒性评级。例如，ISO 10993医疗器械细胞毒性试验允许使用视觉分级量表。本研究比较了生物材料的目视体外细胞毒性评级与定量体外细胞毒性测量，以确定目视评分与定量细胞活力测定之间的相关性水平。评价了代表一系列生物反应性水平的生物材料，包括有机锡聚氯乙烯（PVC;一种已知的细胞毒性材料）、超高分子量聚乙烯（一种已知的非细胞毒性材料）和植入式组织粘合剂。将每种材料与小鼠3 T3成纤维细胞培养物直接接触孵育24 h。使用5分评定量表对材料进行视觉评分;评分员对材料标识不知情。使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑（MTT）比色测定法进行细胞活力的定量测量;同样，测定操作员对材料身份不知情。调查显示，目视细胞毒性评分和定量细胞活力测量之间存在高度相关性; Pearson相关性给出目视细胞毒性评分和活细胞百分比之间的相关系数为0.90。推导出目视细胞毒性评分与活细胞百分比相关的方程。本研究结果对于新型生物材料体外细胞毒性研究的设计和解释具有重要意义。
Medical devices and implanted biomaterials are often assessed for biological reactivity using visual scores of cell-material interactions. In such testing, biomaterials are assigned cytotoxicity ratings based on visual evidence of morphological cellular changes, including cell lysis, rounding, spreading, and proliferation. For example, ISO 10993 cytotoxicity testing of medical devices allows the use of a visual grading scale. The present study compared visual in vitro cytotoxicity ratings to quantitative in vitro cytotoxicity measurements for biomaterials to determine the level of correlation between visual scoring and a quantitative cell viability assay. Biomaterials representing a spectrum of biological reactivity levels were evaluated, including organo-tin polyvinylchloride (PVC; a known cytotoxic material), ultra-high molecular weight polyethylene (a known non-cytotoxic material), and implantable tissue adhesives. Each material was incubated in direct contact with mouse 3T3 fibroblast cell cultures for 24 h. Visual scores were assigned to the materials using a 5-point rating scale; the scorer was blinded to the material identities. Quantitative measurements of cell viability were performed using a 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay; again, the assay operator was blinded to material identities. The investigation revealed a high degree of correlation between visual cytotoxicity ratings and quantitative cell viability measurements; a Pearson's correlation gave a correlation coefficient of 0.90 between the visual cytotoxicity score and the percent viable cells. An equation relating the visual cytotoxicity score and the percent viable cells was derived. The results of this study are significant for the design and interpretation of in vitro cytotoxicity studies of novel biomaterials.