An electron microscopic characterization of classes of synaptic vesicles by means of controlled aldehyde fixation.
An electron microscopic characterization of classes of synaptic vesicles by means of controlled aldehyde fixation.
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DOI：
10.1083/jcb.44.1.115
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发表时间：
1970-01
影响因子：
7.8
通讯作者：
Bodian, D
中科院分区：
文献类型：
--
作者：
Bodian, D
醛固定的变量，可能会影响无颗粒的突触囊泡的形状的检查显示，即使短暂的存储cacodylate缓冲液中的神经组织块，在四氧化锇硬化之前，有一个不寻常的严重扁平化的影响无颗粒囊泡的特定类型。这些是外周胆碱能轴突末梢和某些中枢突触球的囊泡。突触球的类型，现在可以进一步定义的基础上的形状无颗粒突触囊泡在醛固定的控制条件。先前描述的脊髓中的“S”球包含均匀的球状囊泡，其完全抵抗扁平化。先前描述的“F”球包含稍小的无颗粒囊泡，其在醛固定后变平，即使随后在四氧化锇溶液中迅速硬化。第三种类型，以前的特点是具有不规则的圆形无颗粒囊泡后，上述处理，只包含严重扁平囊泡时，四氧化锇硬化之前，甚至简短的洗涤与钠cacodylate缓冲液含有蔗糖。此外，“第三”类型是所有胆碱能外周轴突末梢检查的特征，以及脊髓的大轴体（“L”）突触球。
Examination of variables of aldehyde fixation that may affect the shape of agranular synaptic vesicles has revealed that even brief storage of aldehyde-perfused nervous tissue pieces in cacodylate buffer, prior to hardening in osmium tetroxide, has an unusually severe flattening effect on agranular vesicles of a particular type. These are the vesicles of peripheral cholinergic axon endings, and of certain central synaptic bulbs. Types of synaptic bulbs can now be further defined on the basis of shape of agranular synaptic vesicles under controlled conditions of aldehyde fixation. Previously described "S" bulbs in the spinal cord contain uniformly spheroid vesicles, which are wholly resistant to flattening. Previously described "F" bulbs contain somewhat smaller agranular vesicles that are flattened after aldehyde fixation, even when this is followed by prompt hardening in osmium tetroxide solution. A third type, previously characterized as having irregularly round agranular vesicles after the above treatment, contains only severely flattened vesicles when the osmium tetroxide hardening is preceded by even a brief wash with sodium cacodylate buffer containing sucrose. Moreover, the "third" type is characteristic of all cholinergic peripheral axon endings examined, as well as the large axosomatic ("L") synaptic bulbs of the spinal cord.