EXPRESSION OF DIFFERENTIATIVE TRAITS IN ABSENCE OF CELL-FUSION DURING MYOGENESIS IN CULTURE
EXPRESSION OF DIFFERENTIATIVE TRAITS IN ABSENCE OF CELL-FUSION DURING MYOGENESIS IN CULTURE
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DOI：
10.1016/0045-6039(76)90015-4
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
MOLINARO, M
中科院分区：
文献类型：
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作者：
ADAMO, S;ZANI, B;MOLINARO, M
鸡成肌细胞的融合在低Ca++浓度（0.44 mM）的培养物中受到抑制;肌酸磷酸激酶和肌激酶活性以及肌球蛋白合成和后成肌细胞的外观与进行细胞融合的对照培养物（在1.04 mM Ca++下生长）没有显着差异。当培养第2天后Ca++浓度增加到对照值时，融合发生非常迅速，并且不受放线菌素D或放线菌酮的抑制。用0.06 mM溴脱氧尿苷处理强烈抑制肌酸磷酸激酶活性和肌管形成。去除类似物后细胞融合逆转和肌酸磷酸激酶活性的动力学研究表明，该过程比掺入DNA的溴脱氧尿苷的相对含量的降低慢。所检测的分化特征的表达显然不需要细胞融合。肌管形成的过程似乎意味着2个后续阶段，首先是缓慢的成熟过程，然后是细胞膜的实际融合;前者是Ca++独立的，后者是Ca ++依赖的，不需要RNA或蛋白质合成。
Fusion of chick myoblasts is inhibited in cultures at low Ca++ concentration (0.44 mM); creatine phosphokinase and myokinase activities as well as myosin synthesis and the appearance of post-myoblasts do not significantly differ from those of control cultures (grown at 1.04 mM Ca++) which undergo cell fusion. When Ca++ concentration is increased to the control value after the 2nd day of culture, fusion occurs very rapidly and it is not inhibited by actinomycin D or cycloheximide. Treatment with 0.06 mM bromodeoxyuridine strongly inhibits creatine phosphokinase activity and myotubes formation. The study of the kinetics of reversal of cell fusion and of creatine phosphokinase activity after removal of the analog, shows that this process is slower than the decrease of the relative content of bromodeoxyuridine incorporated into DNA. The expression of the differentiative characters examined apparently does not require cell fusion. The process of myotube formation seems to imply 2 subsequent stages consisting first of a slow maturative process, which is followed by the actual fusion of cell membranes; the former is Ca++ independent, the latter is Ca++ dependent and does not require RNA or protein synthesis.