Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1
Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1
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DOI：
10.3390/biom10050755
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发表时间：
2020-05-01
期刊：
影响因子：
5.5
通讯作者：
Song, Yuanda
中科院分区：
文献类型：
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作者：
Nazir, Yusuf;Halim, Hafiy;Song, Yuanda
在本研究中，8种植物激素从6个不同的类的生长，脂质和二十二碳六烯酸（DHA）的生物合成能力的Aurantiochytrium sp. SW 1（SW 1）的影响进行了评估。激动素（Kinetin，KIN）、茉莉酸（JA）和赤霉素（GA）对SW 1的生长和DHA产量有显著的促进作用，分别比对照提高了16%~ 28%和66%~ 84%。通过响应面法（RSM）评估这三种植物激素的协同效应，表明与单独补充相比，3.6 mg/L GA、2.0 mg/L KIN和20.0 mg/L JA的组合进一步增加了SW 1的生长和DHA产量，分别增加了16%至28%和22%至36%。这些植物激素的协同效应也被证明是时间依赖性的，其中在培养24小时的进料导致与0小时相比，生物量、脂质和DHA生产分别进一步增加15%、26%和35%。应激标记物，抗氧化酶和参与脂肪酸生物合成的关键酶的测定有助于阐明SW 1在不同时间喂养改善生长和DHA产量的潜在机制。与对照组和其他时间点相比，24 h时补充植物激素对降低活性氧（ROS）和丙二醛（MDA）水平具有最大影响，并增强了抗氧化剂（超氧化物歧化酶和过氧化氢酶）和参与脂肪生成的关键代谢酶（苹果酸、葡萄糖-6-磷酸脱氢酶和ATP-柠檬酸裂解酶）。本研究表明，植物激素在提高橙色壶菌生长、脂质和DHA产量方面具有潜在的应用价值。
In the present study, the impact of eight phytohormones from six different classes on the growth, lipid and docosahexaenoic acid (DHA) biosynthetic capacity of Aurantiochytrium sp. SW1 (SW1) was evaluated. Kinetin (KIN), jasmonic acid (JA) and gibberellic acid (GA) significantly enhanced the growth and DHA production of SW1 by 16%-28% and 66%-84% in comparison to the control, respectively. The synergistic effect of these three phytohormones, evaluated by the response surface methodology (RSM), showed that a combination of 3.6 mg/L GA, 2.0 mg/L KIN and 20.0 mg/L JA further increased the growth and DHA production of SW1 by 16% to 28% and 22% to 36%, respectively, in comparison to the individual supplementation. The synergistic effect of these phytohormones was also shown to be time-dependent, where feeding at 24 h of cultivation led to 15%, 26% and 35% further increments in the biomass, lipid and DHA production in comparison to that of 0 h, respectively. The determination of stress markers, antioxidant enzymes and key enzymes involved in fatty acid biosynthesis aided to elucidate the potential mechanism underlying the improvement of growth and DHA production by SW1 at various times of feeding. Supplementation with the phytohormones at 24 h exhibited the maximum impact on reducing the level of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as augmented the antioxidants (superoxide dismutase and catalase) and key metabolic enzymes involved in lipogenesis (malic, glucose-6-phosphate dehydrogenase and ATP-citrate lyase) in comparison to the control and other time points. This study signifies the potential application of phytohormones for improving the growth, lipid and DHA production in Aurantiochytrium spp.