Cell lineage visualisation
Cell lineage visualisation
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DOI：
10.1111/cgf.12614
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发表时间：
2015-06-01
影响因子：
2.5
通讯作者：
Errington, R. J.
中科院分区：
文献类型：
--
作者：
Pretorius, A. J.;Khan, I. A.;Errington, R. J.
细胞谱系描述了细胞群的发育历史，是通过结合延时成像和图像处理产生的。生物医学研究人员研究细胞谱系，以了解细胞分化和抗癌药物的药效作用等基本过程。然而，细胞谱系的解释因其复杂性和视觉分析能力不足而受到阻碍。我们提出了一种细胞谱系交互式可视化的新方法。基于对细胞生物学和活细胞成像方法的理解，我们确定了三个要求：多模态（细胞谱系结合了空间、时间和其他特性）、对称性（与谱系分支结构相关）和同步性（与细胞事件的时间对齐相关）。我们通过将任意数量的谱系的时空行为的视觉总结（包括平均行为的变化）与强调对称性和同步性的存在或不存在的节点链接表示相结合来解决这些问题。我们通过展示一个真实世界的案例研究来说明我们方法的优点，其中确定了抗癌药物拓扑替康的细胞毒性作用。
Cell lineages describe the developmental history of Cell populations and are produced by combining time-lapse imaging and image processing. Biomedical researchers study Cell lineages to understand fundamental processes such as Cell differentiation and the pharmacodynamic action of anticancer agents. Yet, the interpretation of Cell lineages is hindered by their complexity and insufficient capacity for visual analysis. We present a novel approach for interactive visualisation of Cell lineages. Based on an understanding of Cellular biology and live-Cell imaging methodology, we identify three requirements: multimodality (Cell lineages combine spatial, temporal, and other properties), symmetry (related to lineage branching structure), and synchrony (related to temporal alignment of Cellular events). We address these by combining visual summaries of the spatiotemporal behaviour of an arbitrary number of lineages, including variation from average behaviour, with node-link representations that emphasise the presence or absence of symmetry and synchrony. We illustrate the merit of our approach by presenting a real-world case study where the cytotoxic action of the anticancer drug topotecan was determined.