Effect of RNA integrity on uniquely mapped reads in RNA-Seq.
Effect of RNA integrity on uniquely mapped reads in RNA-Seq.
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DOI：
10.1186/1756-0500-7-753
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发表时间：
2014-10-23
影响因子：
1.8
通讯作者：
Knowles JA
中科院分区：
文献类型：
--
作者：
Chen EA;Souaiaia T;Herstein JS;Evgrafov OV;Spitsyna VN;Rebolini DF;Knowles JA
We examined the performance of three RNA-Sequencing library preparation protocols as a function of RNA integrity, comparing gene expressions between heat-degraded samples to their high-quality counterparts. This work is invaluable given the difficulty of obtaining high-quality RNA from tissues, particularly those from individuals with disease phenotypes. With the integrity of total RNA being a critical parameter for RNA-Sequencing analysis, degraded RNA can heavily influence the results of gene expression profiles. We discovered that gene expression read results are influenced by RNA quality when a common library construction protocol is used. These results are based on one technical experiment from a pool of 4 neural progenitor cell lines. The use of alternative protocols can allow samples with a wider range of RNA qualities to be used, facilitating the investigation of disease tissues.
