MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin
MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin
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DOI：
10.1016/j.canlet.2008.08.003
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发表时间：
2009-01-18
期刊：
影响因子：
9.7
通讯作者：
Li, Xin
中科院分区：
文献类型：
--
作者：
Liu, Tao;Tang, Hua;Li, Xin
MicroRNA (miRNA) 可能充当癌基因或肿瘤抑制基因。在这里，我们发现 miR-27a 在人胃腺癌中表达上调。抑制 miR-27a 可抑制胃癌细胞的生长。随后，结合生物信息学和微阵列分析，抑制素被确定为潜在的 miR-27a 靶标。 EGFP报告实验还证实，抑制素的3'非翻译区（3'UTR）携带miR-27a的直接结合位点。在胃癌细胞中敲低 miR-27a 后，抑制素的 mRNA 水平和蛋白水平均升高。 miR-27a 对抑制素的下调可能解释了为什么抑制 miR-27a 可以抑制胃癌细胞的生长，进一步支持 miR-27a 作为癌基因的功能。 (C) 2008 Elsevier Ireland Ltd. 保留所有权利。
MicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we show that miR-27a is up-regulated in human gastric adenocarcinoma. Suppression of miR-27a inhibits gastric cancer cell growth. Subsequently, prohibitin is identified as a potential miR-27a target, combining bioinformatics and microarray analysis. EGFP report experiment also confirms that the 3' untranslated region (3'UTR) of prohibitin carries the directly binding site of miR-27a. After knockdown of miR-27a in gastric cancer cells, mRNA level and protein level of prohibitin are both elevated. Down-regulation of prohibitin by miR-27a may explain why suppression of miR-27a can inhibit gastric cancer cell growth, further supporting that miR-27a functions as an oncogene. (C) 2008 Elsevier Ireland Ltd. All rights reserved.