Activity of an enzyme immobilized on polyelectrolyte multilayers
Activity of an enzyme immobilized on polyelectrolyte multilayers
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固定在聚电解质多层上的酶的活性
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
A. Yaroslavov
中科院分区：
文献类型：
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作者：
A. S. Malinin;A. A. Rakhnyanskaya;A. Bacheva;A. Yaroslavov
采用多层聚电解质吸附技术，将α-胰凝乳酶固定在硅酸硼玻璃微球表面。结果表明，该酶同时吸附在带正电和带负电的表面上，其活性相对于溶液有部分保留。该酶的活性取决于玻璃微球上初步吸附的聚电解质层数和表面的电荷。吸附在带负电荷表面的α-糜蛋白酶的活性比吸附在带正电荷的表面的该酶的活性高四倍。
The immobilization of α-chymotrypsin on the surface of boron silicate glass microspheres is conducted via the technique of multilayer adsorption of polyelectrolytes. It is shown that the enzyme is adsorbed on both positively and negatively charged surfaces and its activity is partially preserved relative to that in solution. The activity of the enzyme depends on the number of polyectrolyte layers preliminarily adsorbed on glass microspheres and on the charge of the surface. The activity of α-chymotrypsin adsorbed on the negatively charged surface is four times higher than the activity of this enzyme adsorbed on a positively charged surface.