Dynamics of MONOPTEROS and PIN-FORMED1 expression during leaf vein pattern formation in Arabidopsis thaliana
Dynamics of MONOPTEROS and PIN-FORMED1 expression during leaf vein pattern formation in Arabidopsis thaliana
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DOI：
10.1111/j.1365-313x.2006.02977.x
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发表时间：
2007-02-01
期刊：
影响因子：
7.2
通讯作者：
Mattsson, Jim
中科院分区：
文献类型：
--
作者：
Wenzel, Carol L.;Schuetz, Mathias;Mattsson, Jim
遗传证据将拟南芥MONOPTEROS（MP）和PIN-FORMED 1（PIN 1）基因与叶脉的图案联系起来。为了阐明它们在这一过程中的潜在功能和相互作用，我们评估了MP和PIN 1在拟南芥叶原基维管图案形成过程中的表达动态。这两个基因在明显的血管分化之前经历了一个动态的表达过程，逐渐细化为一到两个细胞宽的文件。PIN 1的亚细胞分布也从等径细胞中的非极性分布逐渐细化到伸长的原形成层细胞中的强烈极化，并提供了生长素流动的总体方向的指示。我们发现证据表明，MP的表达可以激活生长素曝光和PIN 1以及DR 5：：GUS表达是有缺陷的MP突变体叶片。两者合计的结果表明，一个反馈调节回路，涉及生长素，MP和PIN 1，并提供新的实验支持的渠道生长素流假说。
Genetic evidence links the Arabidopsis MONOPTEROS (MP) and PIN-FORMED1 (PIN1) genes to the patterning of leaf veins. To elucidate their potential functions and interactions in this process, we have assessed the dynamics of MP and PIN1 expression during vascular patterning in Arabidopsis leaf primordia. Both genes undergo a dynamic process of gradual refinement of expression into files one to two cells wide before overt vascular differentiation. The subcellular distribution of PIN1 is also gradually refined from a non-polar distribution in isodiametric cells to strongly polarized in elongated procambial cells and provides an indication of overall directions of auxin flow. We found evidence that MP expression can be activated by auxin exposure and that PIN1 as well as DR5::GUS expression is defective in mp mutant leaves. Taken together the results suggest a feedback regulatory loop that involves auxin, MP and PIN1 and provide novel experimental support for the canalization-of-auxin-flow hypothesis.