Determination of mouse bladder inflammatory response to E-coli lipopolysaccharide
Determination of mouse bladder inflammatory response to E-coli lipopolysaccharide
复制标题
DOI：
10.1007/s002400000114
复制
发表时间：
2000-08-01
影响因子：
--
通讯作者：
Saban, R
中科院分区：
文献类型：
--
作者：
Jerde, TJ;Bjorling, DE;Saban, R
评估与膀胱炎相关的组织学改变的严重程度通常是主观的，而且从一个样本到下一个样本不一致。本研究的目的是建立一种一致的、可重复性的方法来量化脂多糖(LPS)诱导的膀胱炎小鼠模型的组织学变化。雌性C57bk-6J小鼠膀胱内注入脂多糖(n=8)或无热原生理盐水(n=8)。24小时后，处死这些组小鼠和8只未处理的对照组，取出膀胱，用福尔马林固定，苏木精伊红(H&E)染色。膀胱炎指数(BII)是通过回顾组织中的水肿、白细胞浸润和出血来描述的。横断面由一位病理学家以盲法根据所描述的BII目标进行评估。BII法客观分析膀胱炎有效，重复性好。与生理盐水或未经处理的对照组相比，注入内毒素的膀胱炎评分显著增加(n=8，P<；0.05)。这些结果表明，内毒素在膀胱内注射时会引起膀胱炎，并且可以用所述的组织学方法客观地定量小鼠膀胱的炎症。
Evaluation of the severity of histologic changes associated with cystitis is often subjective and inconsistent from one sample to the next. The objective of this study was to establish a consistent, reproducible method to quantify histologic changes in a mouse model of lipopolysaccharide (LPS)-induced cystitis. Either LPS (n = 8) or pyrogen-free saline (n = 8) was instilled intravesically into the bladders of female C57bk-6 J mice. Twenty-four hours later, mice in these groups as well as eight untreated controls were sacrificed and bladders were removed, fixed in formalin, and stained with hematoxylin and eosin (H&E). A bladder inflammatory index (BII) was described by reviewing tissues for edema, leukocyte infiltration, and hemorrhage. Cross-sections were evaluated by a single pathologist in a blinded manner based on the objective BII described. The BII method for objectively analyzing bladder inflammation was effective and reproducible. Bladders instilled with LPS had significantly increased inflammation scores for edema, leukocyte infiltration, and hemorrhage compared with those instilled with saline or untreated controls (n = 8, P < 0.05). These results demonstrate that LPS causes bladder inflammation when instilled intravesically and that inflammation of mouse bladders can be objectively quantified using the histological method described.