The Truffle Microbiome: Species and Geography Effects on Bacteria Associated with Fruiting Bodies of Hypogeous Pezizales
The Truffle Microbiome: Species and Geography Effects on Bacteria Associated with Fruiting Bodies of Hypogeous Pezizales
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DOI：
10.1007/s00248-016-0755-3
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发表时间：
2016-07-01
影响因子：
3.6
通讯作者：
Bonito, Gregory M.
中科院分区：
文献类型：
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作者：
Benucci, Gian Maria Niccolo;Bonito, Gregory M.
在土壤剖面地下产生子实体的真菌通常被称为松露。块菌子实体含有多种多样但知之甚少的细菌、酵母和丝状真菌微生物群落。在这项研究中，我们使用了下一代454扩增子焦磷酸测序的V1和V4区的细菌16 S核糖体DNA（rDNA），以表征和比较松露物种和地理来源对松露微生物组的影响。我们比较了块菌群的glebal组织的8种块菌属于四个不同的属内Pezizales：块茎，Terfezia，Leucangium，和Kalapuya。块菌中的细菌群落以变形菌门、拟杆菌门、放线菌门和厚壁菌门为主。块菌中的细菌丰富度总体上相当低，具有2-23个操作分类单位（OTU）。值得注意的是，我们发现一个单一的慢生根瘤菌OTU是占主导地位的块菌属的物种，无论地理来源，但不是在其他块菌属采样。这项研究提供了有关松露微生物组的相关见解，并提出了有关这些真菌相关细菌财团的招募和功能的问题。
Fungi that produce their fruiting bodies underground within the soil profile are known commonly as truffles. Truffle fruiting bodies harbor a diverse but poorly understood microbial community of bacteria, yeasts, and filamentous fungi. In this study, we used next-generation 454 amplicon pyrosequencing of the V1 and V4 region of the bacterial 16S ribosomal DNA (rDNA) in order to characterize and compare effects of truffle species and geographic origin on the truffle microbiome. We compared truffle microbiomes of the glebal tissue for eight truffle species belonging to four distinct genera within the Pezizales: Tuber, Terfezia, Leucangium, and Kalapuya. The bacterial community within truffles was dominated by Proteobacteria, Bacterioides, Actinobacteria, and Firmicutes. Bacterial richness within truffles was quite low overall, with between 2-23 operational taxonomic units (OTUs). Notably, we found a single Bradyrhizobium OTU to be dominant within truffle species belonging to the genus Tuber, irrespective of geographic origin, but not in other truffle genera sampled. This study offers relevant insights into the truffle microbiome and raises questions concerning the recruitment and function of these fungal-associated bacteria consortia.