SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages.
SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages.
复制标题
脂多糖刺激的腹膜巨噬细胞中 NO 代谢的 SP-D 依赖性调节。
DOI：
10.1007/s10517-009-0525-z
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发表时间：
2009
影响因子：
0.7
通讯作者：
Malyshev,IYu
中科院分区：
文献类型：
--
作者：
Atochina-Vasserman,EN;Abramova,EV;Tomer,Y;Scott,P;Nazarov,VA;Kruglov,SV;Beers,MF;Gow,AJ;Malyshev,IYu
This work was designed to study the role of surfactant protein D in the regulation of NO synthesis by “non-alveolar” microphages. We evaluated whether the effects of surfactant protein D depend on the phenotype of macrophages. In the absence of surfactant protein D, the LPS-induced iNOS response was shown to decrease in macrophages of native and proinflammatory phenotypes by 30%, and in macrophages of the antiinflammatory phenotype (by 63%). Under the influence of lipopolysaccharide in high doses (500 ng/ml), NO2∸production by mouse macrophages without surfactant protein D was reduced in native cells (by 25%), but increased in proinflammatory (by 40%) and antiinflammatory phenotypes (by 12% compared to mouse macrophages with surfactant protein D). Our results suggest that surfactant protein D is involved in the immune response in the whole organism, but not only in the lungs. The effect of surfactant protein D depends on the phenotype of macrophages.
