Small GTPase Rab3A is associated with melanosomes in melanoma cells
Small GTPase Rab3A is associated with melanosomes in melanoma cells
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DOI：
10.1034/j.1600-0749.2000.130505.x
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发表时间：
2000-10-01
期刊：
影响因子：
--
通讯作者：
Ichihashi, M
中科院分区：
文献类型：
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作者：
Araki, K;Horikawa, T;Ichihashi, M
Rab 3A是一个小的鸟苷三磷酸（GTP）结合蛋白，最近已牵连在细胞内囊泡运输和神经递质的分泌在神经元细胞，我们在这里证明，Rab 3A与色素细胞中的黑素体。Rab 3A以及Rab 3A的推定靶蛋白Rabphilin 3A仅在通过蔗糖密度梯度超离心从B16小鼠黑素瘤细胞纯化的黑素体组分中检测到，Tn对比，RabGDP解离抑制剂（GDI），Rab 3A的GDP/GTP交换蛋白，在胞质组分中发现，进一步的研究使用共聚焦激光扫描显微镜和免疫电镜显示，免疫反应Rab 3A。这些结果表明Rab 3A-Rabphilin 3A复合物参与色素细胞中黑素体的胞内转运的可能性，RabGDI调控Rab 3A-Rabphilin 3A复合物。
Rab3A is a small guanosine triphosphate (GTP)-binding protein that has been recently implicated in intracellular vesicle transport and the secretion of neurotransmitters in neuronal cells, We demonstrate here that Rab3A is associated with melanosomes in pigment cells. Rab3A as well as Rabphilin3A, a putative target protein of Rab3A, mere detected in the melanosome fraction, purified from B16 murine melanoma cells by sucrose density gradient ultracentrifugation, Tn contrast, Rab GDP dissociation inhibitor (GDI), a GDP/GTP exchange protein for Rab3A, was found in the cytosol fraction, Further studies using confocal laser scanning microscopy and immunoelectron microscopy revealed that immunoreactive Rab3A. is localized in conjunction with the melanosomal membrane, These results suggest the possibility of involvement of Rab3A-Rabphilin3A complex, regulated by Rab GDI, in the intracellular transport of melanosomes in pigment cells.