PKC-1 acts with the ERK MAPK signaling pathway to regulate Caenorhabditis elegans mechanosensory response.
PKC-1 acts with the ERK MAPK signaling pathway to regulate Caenorhabditis elegans mechanosensory response.
复制标题
PKC-1 与 ERK MAPK 信号通路共同作用，调节秀丽隐杆线虫机械感觉反应。
DOI：
10.1111/j.1601-183x.2010.00667.x
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Hart AC
中科院分区：
文献类型：
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作者：
Hyde R;Corkins ME;Somers GA;Hart AC
在大多数动物中，多个基因编码蛋白激酶C （PKC）蛋白。药理学研究已经揭示了该蛋白家族的许多作用，但特异性PKC蛋白的体内作用和PKC激活的功能靶点尚不清楚。我们发现秀丽隐杆线虫的机械感觉反应需要两个PKC基因PKC -1和tpa-1；详细考察了nPKCε/η同源物pkc-1的作用。pkc-1的功能是成年秀丽隐杆线虫对鼻子触摸的反应所必需的，pkc-1可能在调节运动的中间神经元中起作用，而运动是机械感觉神经元的直接突触靶点。先前的研究已经提出了pkc-1的许多可能靶点；我们的分析表明pkc-1可能通过ERK/MAPK途径起作用。我们发现ERK/MAPK通路的功能是秀丽隐杆线虫机械感觉反应所必需的，并且该通路中至少有一个组分lin-45 Raf在机械感觉回路的中间神经元中起作用。遗传分析表明，这些lin-45和pkc-1共同作用，调节鼻子触摸反应。因此，这些结果在功能上连接了成年秀丽隐杆线虫神经元中的两条保守信号通路，并确定了PKC基因在体内的不同作用。
In most animals, multiple genes encode protein kinase C (PKC) proteins. Pharmacological studies have revealed numerous roles for this protein family, yet the in vivo roles of specific PKC proteins and the functional targets of PKC activation are poorly understood. We find that in C. elegans, two PKC genes, pkc-1 and tpa-1, are required for mechanosensory response; the role of the nPKCε/η ortholog, pkc-1, was examined in detail. pkc-1 function is required for response to nose touch in adult C. elegans and pkc-1 likely acts in the interneurons that regulate locomotion which are direct synaptic targets of mechanosensory neurons. Previous studies have suggested numerous possible targets of pkc-1; our analysis indicates that pkc-1 may act via the ERK/MAPK pathway. We find that ERK/MAPK pathway function is required for mechanosensory response in C. elegans and that at least one component of this pathway, lin-45 Raf, acts in interneurons of the mechanosensory circuit. Genetic analysis indicates that these lin-45 and pkc-1 act together to regulate nose touch response. Thus, these results functionally link two conserved signaling pathways in adult C. elegans neurons and define distinct roles for PKC genes in vivo.