Establishment of novel common marmoset embryonic stem cell lines under various conditions
Establishment of novel common marmoset embryonic stem cell lines under various conditions
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DOI：
10.1016/j.scr.2021.102252
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发表时间：
2021-03-10
影响因子：
1.2
通讯作者：
Sasaki, Erika
中科院分区：
文献类型：
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作者：
Kishimoto, Keiko;Shimada, Akiko;Sasaki, Erika
多能干细胞（PSC），胚胎干细胞（ESC）和诱导的PSC（iPSC）是研究生物体胚胎发育的极好工具，分为幼稚和启动状态。可以通过将幼稚ESC/iPSC注射到植入前胚胎中来产生ESC衍生的生殖系嵌合体个体，并且将引发的人ESC/iPSC转化为幼稚状态提供了对上胚层细胞特征的了解。非人ESC/iPSC是人幼稚ESC/iPSC的替代物，这引起伦理问题。在这项研究中，我们使用普通狨猴（Callithrix jacchus）作为动物模型。自1996年以来，已经建立了16个绒猴ESC系。因为这些ESC系中的大多数是雌性的并且是在>10年前衍生的，所以需要新的ESC，特别是雄性绒猴ESC系。在这里，我们成功地建立了17个新的绒猴胚胎干细胞系，包括6个男性胚胎干细胞系的体外受精（IVF）胚胎和12个胚胎干细胞系在无饲养条件下。这份报告是第一个使用无饲养条件和体外受精植入前囊胚在绒猴中建立ESC系的报告，这些新的ESC系可能有助于未来的非人灵长类ESC研究。
Pluripotent stem cells (PSCs), embryonic stem cells (ESCs), and induced PSCs (iPSCs) are excellent tools for studying embryonic development in organisms and classified into naive and primed states. ESC-derived germline chimera individuals can be produced by injecting naive ESCs/iPSCs into preimplantation embryos, and conversion of primed human ESCs/iPSCs into a naive state provides insights into epiblast cell features. Non-human ESCs/iPSCs are alternatives to human naive ESCs/iPSCs, which elicit ethical issues. In this study, we used the common marmoset (Callithrix jacchus) as an animal model. Since 1996, 16 marmoset ESC lines have been established. Because most of these ESC lines are female and were derived >10 years ago, new ESCs, particularly male marmoset ESC lines, are needed. Here, we successfully established 17 novel marmoset ESC lines, including six male ESC lines from in vitro-fertilized (IVF) embryos and 12 ESC lines under feeder-free conditions. This report is the first to establish ESC lines using feeder-free conditions and IVF preimplantation blastocysts in marmosets, and these novel ESC lines could potentially facilitate future non-human primate ESC studies.