In Vitro Models of Cranial Neural Crest Development toward Toxicity Tests: Frog, Mouse and Human.
In Vitro Models of Cranial Neural Crest Development toward Toxicity Tests: Frog, Mouse and Human.
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用于毒性测试的颅神经嵴发育的体外模型：青蛙、小鼠和人类。
DOI：
10.1111/odi.12523
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Furue MK.
中科院分区：
文献类型：
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作者：
Suga M;Hayashi Y;Furue MK.
在颅面发育过程中，颅神经嵴（NC）来源的间充质细胞迁移到咽弓，并广泛参与神经元、雪旺细胞、平滑肌细胞、成骨细胞、软骨细胞和成牙本质细胞的形成，从而形成颌面部结构。（非洲爪蟾）和小鼠（小家鼠），以了解颅NC发育的细胞和分子机制。最近使用人胚胎干细胞（hESC）和人诱导多能干细胞（hiPSC）的研究已经能够在体外产生人NC细胞（NCC），以提供对人NC发育的见解。了解颅面发育的分子机制将有助于开发新的胚胎毒性试验，并降低颅面区域药物诱导的先天性异常（如唇裂或腭裂）的发生率。在这里，我们回顾了体外从异种推定外胚层（动物帽）、小鼠胚胎干细胞（mESC）和人类多能干细胞（hPSC）中获得NCC的培养方法，并讨论了如何使用这些体外模型来帮助阐明颅面发育的潜在机制并开发预测药物诱导的颅面先天性异常的胚胎毒性测试。
During craniofacial development, cranial neural crest (NC)‐derived mesenchymal cells migrate to pharyngeal arches and contribute extensively to neurons, Schwann cells, smooth muscle cells, osteoblasts, chondrocytes, and odontoblasts, forming maxillofacial structures.In vitromodels using model organism cells, such as African clawed frog (Xenopus Laevis) and mouse (Mus Musculus), were developed to understand cellular and molecular mechanisms of cranial NC development. Recent studies using human embryonic stem cells (hESCs) and human‐induced pluripotent stem cells (hiPSCs) have enabled the generation of human NC cells (NCCs)in vitroto provide insight into human NC development. Understanding molecular mechanisms underlying craniofacial development will contribute to develop novel embryotoxicity tests and to decrease the incidence of drug‐induced congenital anomalies in the craniofacial region, such as cleft lip or cleft palate. Here, we review culture methods to derive NCCsin vitrofromXenopuspresumptive ectoderm (animal caps), mouse embryonic stem cells (mESCs), and human pluripotent stem cells (hPSCs) and discuss how thesein vitromodels can be used to help clarify the mechanisms underlying craniofacial development and for developing embryotoxicity tests predicting drug‐induced congenital anomalies in the craniofacial region.