Differential detection of a surrogate biological threat agent (Bacillus globigii) with a portable surface plasmon resonance biosensor
Differential detection of a surrogate biological threat agent (Bacillus globigii) with a portable surface plasmon resonance biosensor
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DOI：
10.1016/j.bios.2015.11.032
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发表时间：
2016-04-15
影响因子：
12.6
通讯作者：
Schmale, David G., III
中科院分区：
文献类型：
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作者：
Adducci, Benjamin A.;Gruszewski, Hope A.;Schmale, David G., III
需要新的方法和技术来快速准确地检测潜在的生物战剂，如炭疽芽孢杆菌，人类和动物的炭疽病原体。在这里，我们报告了使用便携式表面等离子体共振（SPR）生物传感器（SPIRIT 4.0, Seattle Sensor Systems）检测单独的炭疽芽胞杆菌（B. globigii）模拟物以及与不同种类芽孢杆菌的混合物，以测试非特异性干扰。采用直接捕获法和抗体扩增法确定了球形芽孢杆菌孢子的检出限，并在含有另一种芽孢杆菌的混合样品中检测了球形芽孢杆菌孢子。通过直接捕获浓度为10(7)孢子/mL的globigii（抗bg）包裹传感器，并在浓度为10(5)孢子/mL的浓度下进行二抗扩增。用等浓度（10(7)孢子/mL）的1:1混合液对球状芽孢杆菌孢子进行差异检测，二抗扩增。据我们所知，这是首次在含有至少一种额外芽孢杆菌的混合样品中区分检测到球状芽孢杆菌和SPR的报告，突出了SPR在密切相关物种的混合样品中检测任何目标细菌的潜力。随着便携式仪器的可用性，以准确地检测生物战剂，如炭疽芽胞杆菌，应急人员可以及时实施方案，限制暴露的个人数量。Elsevier B.V.出版
New methods and technology are needed to quickly and accurately detect potential biological warfare agents, such as Bacillus anthracis, causal agent of anthrax in humans and animals. Here, we report the detection of a simulant of B. anthracis (B. globigii) alone and in a mixture with a different species of Bacillus to test non-specific interference using a portable surface plasmon resonance (SPR) biosensor (SPIRIT 4.0, Seattle Sensor Systems). Both direct capture and antibody amplification were used to determine the limit of detection for spores of B. globigii, and to detect spores of B. globigii in a mixed sample containing another Bacillus spp. Spores of B. globigii were detected by anti-B. globigii (anti-Bg) coated sensors by direct capture at a concentration of 10(7) spores/mL, and with a secondary antibody amplification at a concentration of 10(5) spores/mL. Spores of B. globigii were differentially detected in a 1:1 mixture with B. pumilus spores from equal concentrations (10(7) spores/mL) with a secondary antibody amplification. To our knowledge, this is the first report of the differential detection of B. globigii with SPR in a mixed sample containing at least one additional Bacillus spp., highlighting the potential for SPR to detect any target bacterium in a mixed sample of closely related species. With the availability of portable instrumentation to accurately detect biological warfare agents such as B. anthracis, emergency responders can implement protocols in a timely fashion, limiting the amount of exposed individuals. Published by Elsevier B.V.