Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress.
Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress.
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DOI：
10.1007/978-1-4939-7668-3_11
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发表时间：
2018
影响因子：
--
通讯作者：
J. Mano;Md. Sanaullah Biswas
中科院分区：
文献类型：
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作者：
J. Mano;Md. Sanaullah Biswas
活性氧氧化膜脂主要产生脂质过氧化物，由此形成各种羰基，即醛类和酮类。其中，具有羰基共轭C-C双键的化合物具有重要的生物学功能，被称为活性羰基物种（reactive carbonyl species， RCS）。植物细胞中发生的十几种RCS，根据其结构的不同，具有广泛的反应性和生物学效应。最近发现一些RCS可以激活植物中的caspase样蛋白酶，从而引发PCD。介绍了采用常规高效液相色谱法进行RCS综合定量分析的方法。
Oxidation of membrane lipids by reactive oxygen species primarily generates lipid peroxides, from which various carbonyls, i.e., aldehydes and ketones, are formed. Among them, those with a carbonyl-conjugated C-C double bond have significant biological functions and are designated as reactive carbonyl species (RCS). A dozen kinds of RCS occurring in plant cells have a broad spectrum of reactivity and biological effects, depending on the structure. Several RCS have been recently found to activate caspase-like proteases in plants, thereby initiating PCD. Comprehensive and quantitative RCS analysis method using conventional HPLC is illustrated.