Immunomodulatory potential of particular Trichinella spiralis muscle larvae excretory-secretory components
Immunomodulatory potential of particular Trichinella spiralis muscle larvae excretory-secretory components
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DOI：
10.1016/j.ijpara.2016.07.008
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发表时间：
2016-12-01
影响因子：
4
通讯作者：
Gruden-Movsesijan, A.
中科院分区：
文献类型：
--
作者：
Cvetkovic, J.;Sofronic-Milosavljevic, Lj.;Gruden-Movsesijan, A.
旋毛虫肌幼虫排泄分泌抗原可诱导大鼠树突状细胞处于半成熟状态。这可能至少部分是细胞外信号调节激酶1/2（ERK 1/2）瞬时激活的结果。在这里，我们研究了排泄分泌抗原的几种成分的潜力（含有45、49和53 kDa蛋白质和重组Tsp 53的天然级分，代表该级分的组分之一），以证明先前观察到的排泄-分泌抗原对体外树突细胞的作用，其特征在于建立特定表型（非常低的MHC II表达、中等的CD 86表达和显著的ICAM-1表达）和功能特性（促炎细胞因子IL-12 p70的低产生和IL-10和TGF-β的高产生）。由这些成分激活的树突状细胞能够引起幼稚T细胞的增殖及其向Th 2和抗炎反应的极化。所研究的抗原具有与排泄分泌抗原几乎相同的诱导T细胞产生IL-4和IL-10的能力，但未能诱导显著的TGF-β合成。可以得出结论，所研究的排泄分泌抗原组分可以在很大程度上再现完整排泄分泌抗原的免疫调节作用，因此可以被认为是对寄生虫实现的抗炎环境的创建重要的分子。(C)2016年澳大利亚寄生虫学会。由爱思唯尔有限公司出版。保留所有权利。
Excretory-secretory antigens of Trichinella spiralis muscle larvae can induce the semi-matured status of rat dendritic cells. This may at least partly be the consequence of transient activation of extracellular signal-regulated lcinases 1/2 (ERK1 /2). Here we investigated the potential of several components of excretory-secretory antigens (native fraction containing 45, 49 and 53 kDa proteins and recombinant Tsp53, representing one of the constituents of this fraction) to demonstrate previously observed effects of excretory-secretory antigens on dendritic cells in vitro, characterised by establishment of a particular phenotype (very low MHC II expression, moderate CD86 expression and significant ICAM-1 expression) and functional properties (low production of pro-inflammatory cytokine IL-12p70, and high production of IL-10 and TGF-beta). Dendritic cells activated by these components were able to provoke proliferation of naive T cells and their polarisation towards Th2 and anti-inflammatory responses. The investigated antigens had almost the same capacity to induce IL-4 and IL-10 production from T cells as excretory-secretory antigens, but failed to induce significant TGF-beta synthesis. It could be concluded that the investigated excretory-secretory antigens components can largely reproduce the immunomodulatory effects of the complete excretory-secretory antigens and therefore may be considered as molecules important for creation of the anti-inflammatory milieu achieved by the parasite. (C) 2016 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.