Phylogenetic study on CTO primer-amplified ammonia-oxidizing bacteria and β-Proteobacteria associated with rice roots grown in a flooded paddy soil
Phylogenetic study on CTO primer-amplified ammonia-oxidizing bacteria and β-Proteobacteria associated with rice roots grown in a flooded paddy soil
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DOI：
10.1080/00380768.2003.10410330
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发表时间：
2003-03
影响因子：
2
通讯作者：
Makoto Ikenaga;S. Asakawa;Yoshitetsu Muraoka;M. Kimura
中科院分区：
文献类型：
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作者：
Makoto Ikenaga;S. Asakawa;Yoshitetsu Muraoka;M. Kimura
摘要采用聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）和序列分析方法，对水稻不同生育期不同节位根中的氨氧化细菌进行了研究。本研究中使用的根样品在7月2日（分蘖期）、7月21日（最大分蘖期）和9月12日（成熟期）采集三次。结果表明，7月2日和7月21日，在所有的根瘤中均检测到大量细菌16 SrDNA的DGGE条带，而9月12日，在少量根瘤中检测到细菌16 SrDNA的DGGE条带，表明在还原土壤条件下，存在氨氧化细菌。水稻根际氨氧化细菌均属于亚硝化螺旋菌属。此外，该引物还扩增出了大量β-变形菌的16 S rDNA，它们分属于红环菌属（Rhodocyclus）和嗜甲基菌属（Methylophilus）。水稻生育期是影响CTO扩增细菌群落的主要因素。根龄也是影响水稻各生育期群落的一个重要因素。
Abstract Ammonia-oxidizing bacteria associated with rice roots that developed from different nodes at different growth stages were studied by using polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and sequencing analysis. Root samples used in this study were collected three times on July 2 (tillering stage), on July 21 (maximum tillering stage), and on September 12 (ripening stage). The fact that many DGGE bands of bacterial 16S rDNA amplified by the CTO primer set that was originally designed for ammonia-oxidizing β-Proteobacteria, were observed in every nodal root on July 2 and July 21 and in nodal roots with small number on September 12 indicated the presence of ammonia-oxidizing bacteria under reduced soil conditions. All the ammonia-oxidizing bacteria associated with rice roots belonged to Nitrosospira spp. In addition, many 16S rDNA of β-Proteobacteria were also amplified by the CTO primer set, and they belonged to the Rhodocyclus and Methylophilus groups. The growth stage of rice plant was the primary factor affecting CTO-amplified bacterial communities. Root age was also estimated to be an important factor influencing the communities throughout the growth stages of rice plants.