Angiotensin II and Atrial Natriuretic Peptide in the Cow Oviductal Contraction In Vitro: Direct Effect and Local Secretion of Prostaglandins, Endothelin-1, and Angiotensin II
Angiotensin II and Atrial Natriuretic Peptide in the Cow Oviductal Contraction In Vitro: Direct Effect and Local Secretion of Prostaglandins, Endothelin-1, and Angiotensin II
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血管紧张素 II 和心房钠尿肽在体外奶牛输卵管收缩中的作用：前列腺素、内皮素-1 和血管紧张素 II 的直接作用和局部分泌
DOI：
10.1095/biolreprod65.3.799
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发表时间：
2001
影响因子：
--
通讯作者：
Kunitada Sato
中科院分区：
文献类型：
--
作者：
M. Wijayagunawardane;A. Miyamoto;Y. Taquahashi;T. Acosta;M. Nishimura;Kunitada Sato
摘要血管紧张素Ⅱ（Ang Ⅱ）和心钠素（ANP）可能参与动情周期输卵管收缩的局部调节。因此，在体外的影响，血管紧张素II和心钠素对牛输卵管分泌和收缩在卵泡期，排卵后，黄体期进行了研究。应用体外微透析系统（MDS）测定了牛输卵管管腔内前列腺素（PGs）、血管紧张素Ⅱ（Ang Ⅱ）和内皮素-1（ET-1）的释放，并观察了Ang Ⅱ和心钠素（ANP）对这些物质局部分泌的影响。卵泡期和排卵后PGs、ET-1和Ang Ⅱ的基础释放量均高于黄体期（P < 0.05）。在孵育4至8小时之间，通过将Ang II（10 - 6 M）或ANP（10 - 7 M）输注到MDS中进行刺激4小时。在卵泡期和排卵后的输卵管中，ANP的输注增加了Ang II的释放，但不增加ET-1的释放。血管紧张素Ⅱ刺激ET-1的释放。Ang II和ANP均增加PGE 2和PGF 2 α的释放。在收缩研究中，在卵泡期和排卵后阶段，将Ang II（10−7 M）或ANP（10−8 M）直接注入培养基中，可增加输卵管收缩的幅度。相反，这些物质在黄体中期对奶牛输卵管的收缩和分泌没有任何影响。这些结果表明，在围排卵期，血管紧张素Ⅱ和心钠素刺激输卵管的收缩幅度在体外。Ang Ⅱ除了直接作用于输卵管收缩外，还可激活输卵管分泌ET-1和PGs。同样，ANP刺激输卵管分泌PGs和Ang II。因此，总体结果表明，在围排卵期牛输卵管中存在功能性内皮素-血管紧张素-ANP系统，其可调节输卵管收缩以确保配子/胚胎通过输卵管运输的最大效率。
Abstract Angiotensin II (Ang II) and atrial natriuretic peptide (ANP) may be involved in local regulation of the oviductal contraction during the estrous cycle. Thus, the in vitro effects of Ang II and ANP on the secretion and contraction of bovine oviduct during the follicular, postovulatory, and luteal phases were investigated. An in vitro microdialysis system (MDS) was utilized to determine the intraluminal release of prostaglandins (PGs), Ang II, and endothelin-1 (ET-1) from the bovine oviducts as well as to observe the effect of Ang II and ANP on the local secretion of these substances. The basal release of PGs, ET-1, and Ang II was higher (P < 0.05) during the follicular and postovulatory phases than during the luteal phase. Stimulation by infusion of Ang II (10−6 M) or ANP (10−7 M) into the MDS was carried out for 4 h between 4 and 8 h of incubation. In the oviducts from the follicular and postovulatory phases, the infusion of ANP increased the release of Ang II, but not of ET-1. Infusion of Ang II stimulated the release of ET-1. Both Ang II and ANP increased PGE2 and PGF2α release. In the contraction study, direct administration of Ang II (10−7 M) or ANP (10−8 M) into the medium during the follicular and postovulatory phases increased the amplitude of oviductal contraction. In contrast, these substances did not show any effect in the contraction and secretion of oviducts from cows during the midluteal phase. These results indicate that during the periovulatory period, Ang II and ANP stimulate the contractile amplitude of the oviduct in vitro. In addition to their direct action on oviductal contraction, Ang II may activate oviductal secretion of ET-1 and PGs. Likewise, ANP stimulates oviductal secretion of PGs and Ang II. Hence, the overall results suggest the existence of a functional endothelin-angiotensin-ANP system in the bovine oviduct during the periovulatory period, which may regulate the oviductal contraction to ensure maximum efficiency of gamete/embryo transport through the oviduct.
牛输卵管及其在繁殖中的作用：文献综述。
DOI：
--
发表时间：
1991
期刊：
The Cornell veterinarian
影响因子：
--
作者：
Ellington,JE
通讯作者：
Ellington,JE
心钠素抑制培养的内皮细胞产生和分泌内皮素。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hu,RM;Levin,ER;Pedram,A;Frank,HJ
通讯作者：
Frank,HJ
影响因子：
--
作者：
BUHI, WC;ASHWORTH, CJ;ALVAREZ, IM
通讯作者：
ALVAREZ, IM