A procedure for isolation of spontaneous mutants with temperature sensitive synthesis of RNA and/or protein
A procedure for isolation of spontaneous mutants with temperature sensitive synthesis of RNA and/or protein
复制标题
一种通过温度敏感的 RNA 和/或蛋白质合成来分离自发突变体的方法
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
R. Takata
中科院分区：
文献类型：
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作者：
L. Isaksson;S. Sköld;J. Skjöldebrand;R. Takata
本文介绍了一种分离大肠杆菌自发温度敏感突变株的方法。它们被选择为高温下抵抗温度诱导的λ原噬菌体和链霉素加氨苄青霉素或氨苄青霉素加环丝氨酸所施加的联合杀伤作用的存活者。如此获得的突变体在限制性温度下在体内RNA或蛋白质或两者的合成中被阻断。
SummaryA procedure for the isolation of spontaneous temperature sensitive mutants of Escherichia coli has been developed. They are selected as survivors at high temperature against the combined killing effects exerted by a temperature inducible lambda prophage and either streptomycin plus ampillicin or ampicillin plus cycloserine. The mutants so obtained are blocked in vivo in the synthesis of RNA or protein or both at restrictive temperature.