Orthogonal Near-Infrared-II Imaging Enables Spatially Distinguishing Tissues Based on Lanthanide-Doped Nanoprobes
Orthogonal Near-Infrared-II Imaging Enables Spatially Distinguishing Tissues Based on Lanthanide-Doped Nanoprobes
复制标题
正交近红外II成像能够基于镧系元素掺杂的纳米探针在空间上区分组织
DOI：
10.1021/acs.analchem.0c03383
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发表时间：
2020-11-03
影响因子：
7.4
通讯作者：
Zhou, Jing
中科院分区：
文献类型：
--
作者：
Jia, Qi;Ma, Liyi;Zhou, Jing
多通道近红外 (NIR)-II 成像为研究复杂的生物过程提供了更精确、更详细的信息。在研究特定的生物过程时，分离的单信号和多信号是必要的，但传统的多通道 NIR-II 成像方法很难获得。利用镧系离子独特的光学性质，特别是在近红外区域的类原子吸光度和发射光谱方面，本研究合成了两种镧系元素掺杂纳米探针：NaYF4:Gd@NaYF4:Nd@NaYF4 (cssNd)和NaYF4:Gd@NaYF4:Er@NaYF4 (cssEr)。这两个纳米探针分别在 730 和 980 nm 激发下显示正交 NIR-II 发射（cssNd 为 1064 和 1330 nm，cssEr 为 1550 nm）。 cssNd 和 cssEr 用于多通道 NIR-II 成像的可行性在体外得到了证明。在不同的纳米探针管理方法下，成功地进行了具有分离的单信号和多信号的体内多通道NIR-II成像，并且可以在两种不同的激发源下在空间上区分组织。我们的研究结果为具有可分离信号的多通道 NIR-II 成像提供了一种新方法，有望用于精确研究复杂的生物过程。
Multichannel near-infrared (NIR)-II imaging provides more precise and detailed information for studying complex biological processes. When studying specific biological processes, a separated single signal and multisignals are essential but difficult to obtain by traditional multichannel NIR-II imaging methods. Taking advantage of the unique optical properties of lanthanide ions, especially in atom-like absorbance and emission spectroscopy in the NIR region, in this study, we synthesized two lanthanidedoped nanoprobes, NaYF4:Gd@NaYF4:Nd@NaYF4 (cssNd) and NaYF4:Gd@NaYF4:Er@NaYF4 (cssEr). These two nanoprobes show orthogonal NIR-II emissions (1064 and 1330 nm for cssNd and 1550 nm for cssEr) under 730 and 980 nm excitation, respectively. The feasibility of cssNd and cssEr for multichannel NIR-II imaging was proven in vitro. Under different methods of administering the nanoprobes, in vivo multichannel NIR-II imaging with both the separated single signal and multisignals was successfully performed and could spatially distinguish tissues under two different excitation sources. Our results provide a new method for multichannel NIR-II imaging with separable signals, which is promising for precisely studying complex biological processes precisely.