Two-dimensional network formation of cardiac myocytes in agar microculture chip with 1480 nm infrared laser photo-thermal etching
Two-dimensional network formation of cardiac myocytes in agar microculture chip with 1480 nm infrared laser photo-thermal etching
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DOI：
10.1039/b304652d
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发表时间：
2003-01-01
期刊：
影响因子：
6.1
通讯作者：
Yasuda, K
中科院分区：
文献类型：
--
作者：
Kojima, K;Moriguchi, H;Yasuda, K
我们已经开发出一种新的方法，使琼脂微结构用于培养细胞，并允许控制细胞网络模式。该方法利用1480 nm红外聚焦激光束的非接触式三维光热蚀刻，该激光束被水和琼脂凝胶强烈吸收，以形成琼脂微结构的形状。它允许在几分钟内在琼脂层中容易地形成微结构，通过100 mW激光的点加热形成细胞培养孔，并通过100 mm s（-1）、40 mW激光的跟踪形成隧道。我们培养大鼠心肌细胞在相邻的微观结构，并观察到同步跳动后，他们进行了物理接触90分钟。我们的研究结果表明，该系统可以在最短的时间内制造和使用微结构进行细胞网络培养，而无需任何昂贵的微制造设施或复杂的程序。
We have developed a new method that enables agar microstructures to be used to cultivate cells and that allows cell network patterns to be controlled. The method makes use of non-contact three-dimensional photo-thermal etching with a 1480 nm infrared focused laser beam, which is strongly absorbed by water and agar gel, to form the shapes of agar microstructures. It allows microstructures to be easily formed in an agar layer within a few minutes, with cell-culture holes formed by the spot heating of a 100 mW laser and tunnels by the tracing of a 100 mm s(-1), 40 mW laser. We cultivated rat cardiac myocytes in adjacent microstructures and observed synchronized beating in them 90 min after they had made physical contact. Our results indicate that the system can make and use microstructures for cell-network cultivation in a minimal amount of time without any expensive microfabrication facilities or complicated procedures.