Streptavidin-biotin-peroxidase nanocomplex-amplified microfluidics immunoassays for simultaneous detection of inflammatory biomarkers
Streptavidin-biotin-peroxidase nanocomplex-amplified microfluidics immunoassays for simultaneous detection of inflammatory biomarkers
复制标题
链霉亲和素-生物素-过氧化物酶纳米复合物放大微流体免疫分析用于同时检测炎症生物标志物
DOI：
10.1016/j.aca.2017.05.031
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发表时间：
2017
影响因子：
6.2
通讯作者：
Jiang Xingyu
中科院分区：
文献类型：
--
作者：
Wu Jing;Chen Yiping;Yang Mingzhu;Wang Yu;Zhang Cheng;Yang Mo;Sun Jiashu;Xie Mengxia;Jiang Xingyu
Simultaneous, sensitive and quantitative detection of biomarkers in infectious disease is crucial for guiding antimicrobial treatment and predicting prognosis. This work reported an ultrasensitive and quantitative microfluidic immunoassay combined with the streptavidin-biotin-peroxidase (SA-B-HRP) nanocomplex-signal amplification system (MIS) to detect two inflammatory biomarkers, procalcitonin (PCT, for discriminating bacterial infections from nonbacterial infections) and interleukin-6 (IL-6, for monitoring the kinetics of infectious disease) simultaneously. The amplification system was based on the one step self-assembly of SA and B-HRP to form the SA-B-HRP nanocomplex, which effectively amplified the chemiluminescent signals. The linear ranges for PCT and IL-6 detections by MIS were 250–1.28 × 105pg mL−1and 5–1280 pg mL−1, and the limit of detection (LOD) were 48.9 pg mL−1and 1.0 pg mL−1, respectively, both of which were significantly improved compared with microfluidic immunoassays without amplification system (MI). More importantly, PCT and IL-6 in human serum could be simultaneously detected in the same run by MIS, which could greatly improve the detection efficiency and reduce the cost. Given the advantages of high sensitivity, multiplex and quantitative detection, MIS could be potentially applied for detection of biomarkers at low concentration in clinical diagnosis.
