Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples.
Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples.
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DOI：
10.1371/journal.pone.0151240
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Sheppard SK
中科院分区：
文献类型：
--
作者：
Harris LG;Murray S;Pascoe B;Bray J;Meric G;Mageiros L;Wilkinson TS;Jeeves R;Rohde H;Schwarz S;de Lencastre H;Miragaia M;Rolo J;Bowden R;Jolley KA;Maiden MC;Mack D;Sheppard SK
细菌物种包括相关的基因型，其可以显示具有重要临床意义的不同表型。表皮葡萄球菌是医院感染的常见原因，并且对其发病机理至关重要的是其在表面上粘附和形成生物膜的能力，从而调节宿主的免疫应答和抗生素的作用。共栖S.表皮细胞群被认为在涉及粘附和生物膜积累的因素方面不同于与疾病相关的细胞群。我们量化了98 S中生物膜形成的差异。表皮葡萄球菌分离株从各种来源，并研究人口结构的基础上核糖体多位点分型（rMLST）和存在/不存在的基因参与粘附和生物膜形成。所有菌株都能够粘附并形成生物膜在体外生长测定和共聚焦显微镜允许分类为5生物膜形态类型的基础上，其厚度，生物体积和粗糙度。系统发育重建将分离株分为三个独立的分支，与主要疾病相关分支中的分离株在形态型上表现出多样性。在生物膜形态特征中，只有生物膜厚度与分支分布有显著相关性。一些已知的粘附相关基因（aap和sesE）在分离株中的分布显示出与物种克隆框架的显着关联。这些数据挑战了生物膜相关基因，如伊卡操纵子上的基因，是低侵袭性S。epidermidis分离株，并表明表型特征，如粘附和生物膜形成，是不固定的克隆下降，但受到存在的各种基因之间的谱系移动的。
Bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. Staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host’s immune response and antibiotics. Commensal S. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm accumulation. We quantified the differences in biofilm formation in 98 S. epidermidis isolates from various sources, and investigated population structure based on ribosomal multilocus typing (rMLST) and the presence/absence of genes involved in adhesion and biofilm formation. All isolates were able to adhere and form biofilms in in vitro growth assays and confocal microscopy allowed classification into 5 biofilm morphotypes based on their thickness, biovolume and roughness. Phylogenetic reconstruction grouped isolates into three separate clades, with the isolates in the main disease associated clade displaying diversity in morphotype. Of the biofilm morphology characteristics, only biofilm thickness had a significant association with clade distribution. The distribution of some known adhesion-associated genes (aap and sesE) among isolates showed a significant association with the species clonal frame. These data challenge the assumption that biofilm-associated genes, such as those on the ica operon, are genetic markers for less invasive S. epidermidis isolates, and suggest that phenotypic characteristics, such as adhesion and biofilm formation, are not fixed by clonal descent but are influenced by the presence of various genes that are mobile among lineages.